马钱子和马钱子果实甲醇提取物对小鼠睡眠调节作用的评价

M. Sani, F. Khan, D. Sani, MM Sulaiman, E. Nwasor, A. Hassan
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摘要

本研究旨在评价马钱子果和马钱子果对瑞士白化小鼠的睡眠调节作用。采用地西泮诱导小鼠睡眠模型,研究其提取物对中枢神经系统的抑制作用。这些老鼠被分成四组,每组6只。试验组分别以250、500、1000 mg/kg体重腹腔注射马钱子提取物和马钱子果实提取物。对照组给予蒸馏水10 ml/kg。地西泮(Ranbaxy®,印度)作为标准药物,在初始治疗后30分钟给药。两种提取物的致死剂量(LD50)均大于5000mg /kg。马钱子甲醇提取物和马钱子果实甲醇提取物对大鼠睡眠的影响结果表明,与对照组相比,分别给药250、500和1000 mg/kg的马钱子甲醇提取物对大鼠睡眠的影响不显著(p >0.05)。用马钱子果实提取物治疗的组的睡眠开始显示出剂量依赖性的减少模式。研究还显示,与对照组相比,接受这两种提取物治疗的所有实验组的睡眠时间都更长。250mg /kg、500mg /kg和1000mg /kg组与对照组相比,地西泮诱导睡眠时间显著延长(p <0.05),且呈剂量依赖性。1000 mg/kg马钱子提取物处理组与对照组相比差异显著(p <0.05)。总之,本研究中观察到的高LD50表明这两种提取物是相对安全的,并且含有有希望的生物活性成分,可以调节小鼠的睡眠。
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Evaluation of the sleep modulating effects of methanolic extracts of Strychnos spinosa and Strychnos innocua fruits in mice
This study aimed to evaluate the sleep modulating properties of Strychnos spinosa and Strychnos innocua fruits in Swiss albino mice. The depressant effect of the extracts on the central nervous system was studied using a diazepam-induced sleep model in mice. The mice were divided into four groups of six mice each. The test groups received Strychnos spinosa and Strychnos innocua fruits extracts at the doses of 250, 500, and 1000 mg/kg body weight intraperitoneally. In contrast, the control group received distilled water at 10 ml/kg. Diazepam (Ranbaxy®, India) was used as a standard drug and administered 30 minutes after the initial treatment. The lethal doses (LD50) of the two extracts were estimated to be >5000 mg/kg. The results obtained from the onset of sleep of rats administered methanol extracts of Strychnos spinosa, and Strychnos innocua fruits showed that the extracts at 250, 500, and 1000 mg/kg, respectively, did not significantly (p >0.05) alter the onset of sleep when compared with the control. The onset of sleep in the groups treated with Strychnos spinosa fruit extracts indicated a dose-dependent decrease pattern. The study also revealed a prolonged duration of sleep in all the experimental groups treated with the two extracts compared to the control group. The treated groups of 250, 500 and 1000 mg/kg significantly (p <0.05) prolonged the duration of diazepam-induced sleep in a dose-dependent manner when compared to the control group. The group treated with 1000 mg/kg of Strychnos innocua fruit extract was significant (p <0.05) compared to the control group. In conclusion, the high LD50 observed in this study suggests that the two extracts are relatively safe and contain promising bioactive ingredients that cause sleep modulation in mice.
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