{"title":"在大蒜-青蒿素治疗后,支持伯氏疟原虫感染小鼠体内抗疟活性的新蛋白设置","authors":"V. Govindan, P. K. Murthy","doi":"10.9734/ijpr/2022/v9i430235","DOIUrl":null,"url":null,"abstract":"Many malaria endemic nations are pursuing malaria elimination and these technical challenges require the development of integrated approaches, among which safe and effective malaria vaccines could be a crucial tool. Due to non-availability of malaria vaccine, the control efforts rely heavily on treatment with new antimalarial agents preferably acting on newer targets. In this study, the protected serum proteomics after garlic and arteether combination treatment of P.berghei infected mice has been analyzed by western blotting. One of the identified host parasites specific proteins, peptidyl-prolyl-cis-trans isomerase A (PPIA) is known to catalyze the interconversion of the cis and trans and mediate certain protein folding events both in in vitro and in vivo conditions. This study hypothesizes that, overexpressed PPIA might lead to misfold of the parasite protein which are needed for parasite multiplication and in turn lead to the parasite death or in the protection of combination drug treated samples.","PeriodicalId":129993,"journal":{"name":"International Journal of Pathogen Research","volume":"126 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"New Protein Settings to Support in vivo Antimalarial Activity in Plasmodium berghei Infected Mice after Garlic-Arteether Therapy\",\"authors\":\"V. Govindan, P. K. Murthy\",\"doi\":\"10.9734/ijpr/2022/v9i430235\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Many malaria endemic nations are pursuing malaria elimination and these technical challenges require the development of integrated approaches, among which safe and effective malaria vaccines could be a crucial tool. Due to non-availability of malaria vaccine, the control efforts rely heavily on treatment with new antimalarial agents preferably acting on newer targets. In this study, the protected serum proteomics after garlic and arteether combination treatment of P.berghei infected mice has been analyzed by western blotting. One of the identified host parasites specific proteins, peptidyl-prolyl-cis-trans isomerase A (PPIA) is known to catalyze the interconversion of the cis and trans and mediate certain protein folding events both in in vitro and in vivo conditions. This study hypothesizes that, overexpressed PPIA might lead to misfold of the parasite protein which are needed for parasite multiplication and in turn lead to the parasite death or in the protection of combination drug treated samples.\",\"PeriodicalId\":129993,\"journal\":{\"name\":\"International Journal of Pathogen Research\",\"volume\":\"126 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-06-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Pathogen Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9734/ijpr/2022/v9i430235\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Pathogen Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/ijpr/2022/v9i430235","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
New Protein Settings to Support in vivo Antimalarial Activity in Plasmodium berghei Infected Mice after Garlic-Arteether Therapy
Many malaria endemic nations are pursuing malaria elimination and these technical challenges require the development of integrated approaches, among which safe and effective malaria vaccines could be a crucial tool. Due to non-availability of malaria vaccine, the control efforts rely heavily on treatment with new antimalarial agents preferably acting on newer targets. In this study, the protected serum proteomics after garlic and arteether combination treatment of P.berghei infected mice has been analyzed by western blotting. One of the identified host parasites specific proteins, peptidyl-prolyl-cis-trans isomerase A (PPIA) is known to catalyze the interconversion of the cis and trans and mediate certain protein folding events both in in vitro and in vivo conditions. This study hypothesizes that, overexpressed PPIA might lead to misfold of the parasite protein which are needed for parasite multiplication and in turn lead to the parasite death or in the protection of combination drug treated samples.