流感病毒感染引起细胞膜改变的分析

Takuro Takahata, S. Kume, H. Miyoshi, T. Sugiura, A. Honda
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引用次数: 0

摘要

流感病毒感染引起宿主细胞的各种变化。在流感病毒感染初期,病毒基因组RNA通过RNA依赖的RNA聚合酶转录,利用自身的核酸内切酶裂解宿主盖帽RNA,引发mRNA合成。病毒蛋白NS1表达后,NS1与CPSF结合,抑制宿主mRNA的polyA添加(1)。最终流感病毒感染降低宿主细胞蛋白表达。而病毒基因组转录后,病毒膜蛋白HA、NA和M1表达并定位在细胞膜上。这一现象被认为是引起细胞膜刚度的原因。我们采用光学激光和胶原蛋白包覆珠对细胞膜刚度进行了测量。有趣的是,在流感病毒感染后4小时,细胞膜硬度发生了变化,细胞膜上的整合素水平也有所下降。
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Analysis of cellular membrane changing induced by influenza virus infection
Influenza virus infection induced various changing of the host cell. At the beginning of influenza virus infection, the transcription from viral genome RNA by RNA-dependent RNA polymerase took place by cleavage of host capped RNA using own endonuclease for priming of mRNA synthesis. After the viral protein NS1 expression, NS1 binds to CPSF lead to inhibit polyA addition of host mRNA (1). Finally the influenza virus infection reduced host cell protein expression. While after viral genome transcription, viral membrane protein HA, NA and M1 expressed and localized on the cellular membrane. It is thought that this phenomenon induced the cellular membrane stiffness. We performed to measure the stiffness of cellular membrane using optical laser and collagen coated-beads. Interestingly at 4 hrs after influenza virus infection, the membrane stiffness changed and also the level of integrin in the cellular membrane was decreased.
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