原位ATP定量分析系统“IISA-ATP”的研制

N. Fukuzawa, T. Fukuba, T. Fujii
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引用次数: 0

摘要

本研究开发了一种集成的原位ATP浓度分析仪(IISA-ATP)。本研究提出了具有细胞裂解、细胞外ATP消除、荧光素-荧光素酶反应定量ATP和原位校准功能的IISA-ATP的概念。采用微加工技术,研制了一种基于PDMS的光学检测微流控芯片的简化原型。通过对样机的评估,成功探测到10-10 M范围内的ATP
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Development of in situ ATP quantitative analysis system "IISA-ATP"
An integrated in situ analyzer for ATP concentration (IISA-ATP) has been developed in this study. Concept of IISA-ATP that has function for cell lysis, extracellular ATP elimination, ATP quantification with luciferin-luciferase reaction and in situ calibration is proposed in this study. A simplified prototype of PDMS based microfluidic chip that has a coiled microchannel pattern for optical detection is developed with micro fabrication technique. As a result of evaluation of the prototype, more than 10-10 M range of ATP was successfully detected
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