{"title":"【全自动测定血清总胆固醇的酶切法(作者译)】。","authors":"J Ziegenhorn","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A simple kinetic method for the automated determination of total serum cholesterol was developed using cholesterol esterase, cholesterol oxidase, catalase and the color reaction according to Hantzsch. The procedure has been adapted to the Braun SysteMatik, Greiner GSA II, Eppendorf 5032, Lars Ljungberg Autolab, Perkin-Elmer C4 and Perkin-Elmer C4B analyzers. The variants of the method yielded satisfactory results with regard of precision, accuracy and sensitivity to interferences.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 3","pages":"109-15"},"PeriodicalIF":0.0000,"publicationDate":"1975-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Ezymatic method for the automated determination of total serum cholesterol (author's transl)].\",\"authors\":\"J Ziegenhorn\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A simple kinetic method for the automated determination of total serum cholesterol was developed using cholesterol esterase, cholesterol oxidase, catalase and the color reaction according to Hantzsch. The procedure has been adapted to the Braun SysteMatik, Greiner GSA II, Eppendorf 5032, Lars Ljungberg Autolab, Perkin-Elmer C4 and Perkin-Elmer C4B analyzers. The variants of the method yielded satisfactory results with regard of precision, accuracy and sensitivity to interferences.</p>\",\"PeriodicalId\":23822,\"journal\":{\"name\":\"Zeitschrift fur klinische Chemie und klinische Biochemie\",\"volume\":\"13 3\",\"pages\":\"109-15\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1975-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift fur klinische Chemie und klinische Biochemie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur klinische Chemie und klinische Biochemie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
根据Hantzsch理论，利用胆固醇酯酶、胆固醇氧化酶、过氧化氢酶和显色反应，建立了一种简单的自动测定血清总胆固醇的动力学方法。该程序已适用于Braun SysteMatik, Greiner GSA II, Eppendorf 5032, Lars Ljungberg Autolab, perkins - elmer C4和perkins - elmer C4B分析仪。该方法的各种变体在精密度、准确度和对干扰的敏感性方面都取得了令人满意的结果。
[Ezymatic method for the automated determination of total serum cholesterol (author's transl)].
A simple kinetic method for the automated determination of total serum cholesterol was developed using cholesterol esterase, cholesterol oxidase, catalase and the color reaction according to Hantzsch. The procedure has been adapted to the Braun SysteMatik, Greiner GSA II, Eppendorf 5032, Lars Ljungberg Autolab, Perkin-Elmer C4 and Perkin-Elmer C4B analyzers. The variants of the method yielded satisfactory results with regard of precision, accuracy and sensitivity to interferences.
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