牛松果体分泌的一些物质及其体外抗促性腺功能的鉴定。

P B Gradwell, E J Candy, R B Symington
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引用次数: 0

摘要

为了阐明大脑骨骺产生的抗促性腺激素物质的化学成分,100个牛松果体在克雷布斯-林格缓冲液中孵育,孵育后用乙酸乙酯和水提取物处理。采用荧光硅胶板薄层色谱法,从松果体培养液和两种提取物中鉴定出5种吲哚,分离出6种未知物质。在培养液和水萃取物中检测到多肽的存在。以牛腺垂体组织中促黄体生成素(LH)的产生为最终参数,用连续流体外系统检测了培养物和两种提取物的抗促性腺激素活性。所有的三个部分被发现在某种程度上产生相似的黄体生成素。为了区分由吲哚和松果体分泌的吲哚/蛋白质成分引起的抗促性腺活性,我们对90个牛松果体进行了匀浆,并在存在或不存在胰蛋白酶和胰蛋白酶抑制剂的情况下将匀浆在体外连续流动系统中孵育。关于吲哚和吲哚/蛋白组分在松果体抗促性腺活性中所起的作用,结果尚无定论。
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Identification of some substances secreted by the bovine pineal body and their antigonadotrophic activity in an in vitro system.

To aid elucidation of the chemical composition of the antigonadotrophic substances produced by the epiphysis cerebri, 100 bovine pineal bodies were incubated in Krebs-Ringer buffer and the incubate treated to give an ethyl acetate and an aqueous extract. Using thin layer chromatography on fluorescent silica gel plates, 5 indoles were identified and 6 unknown substances isolated from the pineal incubate and from both extracts. The presence of peptides was detected in the incubate and in the aqueous extract. The antigonadotrophic activity of the incubate and of the two extracts was examined using a continuous flow in vitro system with the production of luteinizing hormone (LH) from bovine adenohypophysial tissue as the final parameter. All three fractions were found to result in a somewhat similar production of LH. To distinguish between the antigonadotrophic activity attributable to the indole and the indole/protein components of the pineal secretion, 90 bovine pineal bodies were homogenized and the homogenate incubated in the continuous flow in vitro system in the presence or absence of trypsin and trypsin-inhibitor. Results were inconclusive regarding the role played by the indole and indole/protein fractions in the antigonadotrophic activity of the pineal body.

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