去势后,发情期雄性小鼠上颌下唾液腺淀粉酶活性的组织化学变化。

M Shear, F Barbakow, G King
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摘要

小鼠上颌下腺的两性二态性是有充分文献记载的。主要区别在于颗粒状的卷曲小管(gct),成年男性大,而成年女性小且少。先前的研究表明,淀粉酶在组织化学上定位于这些gct;去势后,gct的大小和数量在组织学上都有所减少从生物化学角度来看,淀粉酶活性下降了大约50%。本研究将74只60日龄雄性小鼠分为6组。动物们禁食一夜。在全身麻醉下,切除左侧上颌下腺体,液氮冷冻后进行组织化学处理。然后,这些动物被阉割并放回笼子。在2、4、6、8、10和12周后,在相同条件下切除另一个颌下腺,然后杀死动物。两个腺体的新鲜冷冻切片采用淀粉底物膜技术进行淀粉酶的组织化学演示。相邻切片行HE染色。2周的大鼠表现出轻微但明确的gct大小和数量减少和淀粉酶活性的平行降低。这在第4周和第6周时更为明显。然而,在4周的动物中,这种变化并不一致,在一些动物中比在其他动物中更明显。在6周、8周和10周大的动物身上也观察到了同样的现象,其中一些动物的上颌骨下有相当大的G.C.T.萎缩,而另一些动物的萎缩程度则小得多。在许多12周大的动物中,几乎没有gct萎缩的迹象。这些发现证实了其他研究表明可能存在一些gct再生,可能是由于其他内分泌腺如甲状腺、肾上腺和垂体的影响。
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Histochemically demonstrable changes in amylase activity in submaxillary salivary glands of puberal male mice following castration.

The sexual dimorphism in mouse submaxillary glands is well documented. The main difference is seen in the granular convoluted tubules (G.C.T.) which in the adult male are large and in the adult female are small and fewer. Previous studies have shown that amylase is localized histochemically to these G.C.T.'s; that following castration there is a histological reduction in size and number of the G.C.T.'s and a roughly 50 per cent decrease in amylase activity demonstrable biochemically. In the present study 74 male mice aged 60 days were divided in six groups. The animals were fasted overnight. Under general anaesthesia, the submaxillary glands on the left side were removed and frozen in liquid nitrogen for subsequent histochemistry. The animals were then castrated and returned to their cages. At periods of 2, 4, 6, 8, 10 and 12 weeks later, the other submaxillary gland was removed under the same conditions, and the animals were then killed. Fresh frozen cryostat sections of both glands were subjected to the starch substrate film technique for the histochemical demonstration of amylase. Adjacent sections were stained with HE. The 2-week animals showed a slight but define reduction in size and number of G.C.T.'s and a parallel reduction in amylase activity. This was more pronounced by 4 and 6 weeks. In the 4-week animals, however, the changes were not consistent, being more pronounced in some animals than in others. This same phenomenon was observed in the 6-, 8- and 10-week animals, in some of whom there was considerable G.C.T. atrophy in the submaxillaries and in others very much less. In many of the 12-week animals there was very little evidence of G.C.T. atrophy. These findings confirmed other studies which suggested that there may be some regeneration of G.C.T's, possibly as a result of influence of other endocrine glands such as thyroid, adrenal and pituitary.

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