{"title":"[以草酰乙酸为底物的猪心脏苹果酸脱氢酶的Km[作者译]。","authors":"H U Bergmeyer, G Rozalskis","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Km is necessary to calculate the conditions for indicator reactions in coupled enzymic assays. When malate dehydrogenase is used as an indicator enzyme for the assay of aspartate aminotransferase activity, its Km in relation to oxaloacetate is needed. Km (oxaloacetate) of commercially available mitochondrial malate dehydrogenase from pig heart was determined as Km equals 1.65 x 10(-5) mol/1 using the measurement conditions for aspartate aminotransferase according to the preliminary recommendations of the IFCC.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 11","pages":"509"},"PeriodicalIF":0.0000,"publicationDate":"1975-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[The Km of malate dehydrogenase from pig heart with oxaloacetate as substrate (author's transl)].\",\"authors\":\"H U Bergmeyer, G Rozalskis\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Km is necessary to calculate the conditions for indicator reactions in coupled enzymic assays. When malate dehydrogenase is used as an indicator enzyme for the assay of aspartate aminotransferase activity, its Km in relation to oxaloacetate is needed. Km (oxaloacetate) of commercially available mitochondrial malate dehydrogenase from pig heart was determined as Km equals 1.65 x 10(-5) mol/1 using the measurement conditions for aspartate aminotransferase according to the preliminary recommendations of the IFCC.</p>\",\"PeriodicalId\":23822,\"journal\":{\"name\":\"Zeitschrift fur klinische Chemie und klinische Biochemie\",\"volume\":\"13 11\",\"pages\":\"509\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1975-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift fur klinische Chemie und klinische Biochemie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur klinische Chemie und klinische Biochemie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
Km是计算偶联酶测定中指示反应条件所必需的。当苹果酸脱氢酶作为指示酶用于测定天冬氨酸转氨酶活性时，需要其与草酰乙酸的Km。根据IFCC的初步建议，使用天冬氨酸转氨酶的测量条件，测定猪心脏中市售线粒体苹果酸脱氢酶的Km(草酰乙酸)为Km = 1.65 x 10(-5) mol/1。
[The Km of malate dehydrogenase from pig heart with oxaloacetate as substrate (author's transl)].
Km is necessary to calculate the conditions for indicator reactions in coupled enzymic assays. When malate dehydrogenase is used as an indicator enzyme for the assay of aspartate aminotransferase activity, its Km in relation to oxaloacetate is needed. Km (oxaloacetate) of commercially available mitochondrial malate dehydrogenase from pig heart was determined as Km equals 1.65 x 10(-5) mol/1 using the measurement conditions for aspartate aminotransferase according to the preliminary recommendations of the IFCC.
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