北巴厘水域太阳鱼的首次分子鉴定

I. M. O. Riawan, G. I. Setiabudi, I. M. Merdana, I. P. M. Mariasa, Kadek Teguh Wirasastra
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引用次数: 2

摘要

北巴厘岛搁浅的太阳鱼,我们收集了整个身体来做分子鉴定。采用聚合酶链反应(PCR)法在d环位点(控制区)扩增样品。PCR使用的引物为H16498作为主前(正)引物,L15812作为反向引物。在GenBank上与Mola ramsayi(登录号为AY940824)比对后,相似性值为95%,核苷酸缺口仅为1%。利用部分序列mtDNA鉴定出的搁浅翻车鱼与翻车鱼属同一物种。
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First Molecular Identification of Sunfish in North Bali Water
Stranded Sunfish in North Bali with full body we collect to do molecular identification. Samples were amplified at the d-loop locus (control region) using the PCR (Polymerase Chain Reaction) method. Primers used in PCR are H16498 as primary front (forward) and L15812 as reverse primer. Similarity value of 95% after alignment with Mola ramsayi (accession number accession AY940824) on GenBank, and the gaps of the nucleotide just 1%. The stranded sunfish identified using partial sequence mtDNA is the same species as the species Mola ramsayi.
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