HSP70 and HSP70 mRNA localization in heat-stressed tissues of broilers

The localization of heat shock protein 70 (HSP70) and HSP70 mRNA in the heart, liver, lung, kidney, spleen, thymus and cloacal bursa in broilers that were heat stressed for 6 h was conducted using immunohistochemistry and in situ hybridization techniques. Positive HSP70 mRNA signals were detected in the liver and lung, especially in the vessel walls. A weak presence was found in the myocardial cells. No significant signals were observed in spleen, thymus and cloacal bursa. HSP70 was observed in the vessel walls of all investigated broiler tissues. Localizations of HSP70 and HSP70 mRNA suggest that HSP70 could be correlated with cardiovascular function.