蛋白质测序的质量平衡策略。应用于具有扩展DNPNNP重复基序的蛋白质。

International journal of peptide and protein research Pub Date : 1992-12-01

S Hua, M M Vestling, C M Murphy, D K Bryant, J J Height, C Fenselau, J Theibert, J H Collins

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引用次数: 0

摘要

质量平衡策略的价值在金黄色葡萄球菌V8蛋白酶的序列测定中得到了体现。毛细管电泳，电喷雾质谱，高效串联质谱，以及蛋白质水解和Edman降解。羧基端含有17个不规则重复的三联基序NNP和DNP单元，这对任何涉及水解肽的定位、测序和重叠的策略都是一个挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Mass balance strategy for protein sequencing. Application to a protein with an extended DNPNNP repeating motif.

The value of the mass balance strategy is illustrated in the sequence determination of S. aureus V8 protease. Capillary electrophoresis, electrospray mass spectrometry, and high performance tandem mass spectrometry are used as well as proteolysis and Edman degradation. The carboxy terminus is found to contain 17 irregularly repeating units of the triptych motifs NNP and DNP, which provide a challenge to any strategy involving mapping, sequencing, and overlapping of hydrolytic peptides.

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International journal of peptide and protein research

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