Methods for analyzing next-generation sequencing data X. Registration to DDBJ through Mass Submission System

The International Nucleotide Sequence Database Collaboration (INSDC) has maintained a primary sequence database that collects experimentally-determined nucleotide sequence data directly from researchers. Now data deposition to the INSDC is mandatory for research publication at most of the scientific journals. However, the procedure to deposit data to the INSDC is a big burden, especially for those who are not familiar with computer skills. Recently, we have developed a genome annotation pipeline DFAST, which also assists data deposition to the INSDC. In this article, we show the instruction to deposit annotated genome sequence data using the DFAST web service. Supplementary materials are available at our web site, http://www.iu.a.u-tokyo.ac.jp/~kadota/r_seq.html#about_book_ JSLAB.