N P Balaban, M R Sharipova, V I Vershinina, L V Znamenskaia, E B Chernokal'skaia, E R Romakhina, T I Volkova, S V Lopatnev
{"title":"[从大肠杆菌重组菌株中分离高纯度细菌核糖核酸酶]。","authors":"N P Balaban, M R Sharipova, V I Vershinina, L V Znamenskaia, E B Chernokal'skaia, E R Romakhina, T I Volkova, S V Lopatnev","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Active and inactive highly purified extracellular alkaline ribonucleases (barnase) from recombinant Escherichia coli have been prepared according to the industrial technology of Bacillus intermedius ribonuclease purification. Electrophoretic parameters and ultraviolet spectra characterize these preparations as homogeneous proteins. Immunochemical test system of identification of inactive RNAse for its purification has been worked out.</p>","PeriodicalId":18920,"journal":{"name":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","volume":" 4","pages":"68-74"},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[The isolation of highly purified bacillary ribonucleases from recombinant strains of Escherichia coli].\",\"authors\":\"N P Balaban, M R Sharipova, V I Vershinina, L V Znamenskaia, E B Chernokal'skaia, E R Romakhina, T I Volkova, S V Lopatnev\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Active and inactive highly purified extracellular alkaline ribonucleases (barnase) from recombinant Escherichia coli have been prepared according to the industrial technology of Bacillus intermedius ribonuclease purification. Electrophoretic parameters and ultraviolet spectra characterize these preparations as homogeneous proteins. Immunochemical test system of identification of inactive RNAse for its purification has been worked out.</p>\",\"PeriodicalId\":18920,\"journal\":{\"name\":\"Nauchnye doklady vysshei shkoly. Biologicheskie nauki\",\"volume\":\" 4\",\"pages\":\"68-74\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nauchnye doklady vysshei shkoly. Biologicheskie nauki\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nauchnye doklady vysshei shkoly. Biologicheskie nauki","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[The isolation of highly purified bacillary ribonucleases from recombinant strains of Escherichia coli].
Active and inactive highly purified extracellular alkaline ribonucleases (barnase) from recombinant Escherichia coli have been prepared according to the industrial technology of Bacillus intermedius ribonuclease purification. Electrophoretic parameters and ultraviolet spectra characterize these preparations as homogeneous proteins. Immunochemical test system of identification of inactive RNAse for its purification has been worked out.
分享
京公网安备 11010802042870号
京ICP备2023020795号-1
求助内容：
应助结果提醒方式：
扫码关注我们
