Katherine Sheldon , Reuben Baumal , Alexander Marks
{"title":"利用共价单克隆抗体-链亲和素偶联物靶向[111In]生物细胞素对培养的卵巢腺癌细胞的靶向作用","authors":"Katherine Sheldon , Reuben Baumal , Alexander Marks","doi":"10.1016/0883-2889(92)90015-7","DOIUrl":null,"url":null,"abstract":"<div><p>Three monoclonal antibodies (mAb) directed against the human ovarian adenocarcinoma cell line HEY, were substituted with maleimide and covalently bonded to thiolated streptavidin. The conjugates were separated from unreacted reagents by successive affinity chromatography on protein A—Sepharose and iminobiotin columns. Purified conjugates consisted of an immunoglobulin (Ig) monomer bound to a streptavidin tetramer through a covalent bond between the Ig molecule and one of the streptavidin subunits. The conjugates were able to specifically target [<sup>111</sup>In]biocytin to HEY cells <em>in vitro</em> in the presence of human serum and ascitic fluid from ovarian cancer patients.</p></div>","PeriodicalId":14288,"journal":{"name":"International Journal of Radiation Applications and Instrumentation. Part A. Applied Radiation and Isotopes","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0883-2889(92)90015-7","citationCount":"2","resultStr":"{\"title\":\"Targeting of [111In]biocytin to cultured ovarian adenocarcinoma cells using covalent monoclonal antibody—Streptavidin conjugates\",\"authors\":\"Katherine Sheldon , Reuben Baumal , Alexander Marks\",\"doi\":\"10.1016/0883-2889(92)90015-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Three monoclonal antibodies (mAb) directed against the human ovarian adenocarcinoma cell line HEY, were substituted with maleimide and covalently bonded to thiolated streptavidin. The conjugates were separated from unreacted reagents by successive affinity chromatography on protein A—Sepharose and iminobiotin columns. Purified conjugates consisted of an immunoglobulin (Ig) monomer bound to a streptavidin tetramer through a covalent bond between the Ig molecule and one of the streptavidin subunits. The conjugates were able to specifically target [<sup>111</sup>In]biocytin to HEY cells <em>in vitro</em> in the presence of human serum and ascitic fluid from ovarian cancer patients.</p></div>\",\"PeriodicalId\":14288,\"journal\":{\"name\":\"International Journal of Radiation Applications and Instrumentation. Part A. Applied Radiation and Isotopes\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0883-2889(92)90015-7\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Radiation Applications and Instrumentation. Part A. Applied Radiation and Isotopes\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0883288992900157\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Radiation Applications and Instrumentation. Part A. Applied Radiation and Isotopes","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0883288992900157","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Targeting of [111In]biocytin to cultured ovarian adenocarcinoma cells using covalent monoclonal antibody—Streptavidin conjugates
Three monoclonal antibodies (mAb) directed against the human ovarian adenocarcinoma cell line HEY, were substituted with maleimide and covalently bonded to thiolated streptavidin. The conjugates were separated from unreacted reagents by successive affinity chromatography on protein A—Sepharose and iminobiotin columns. Purified conjugates consisted of an immunoglobulin (Ig) monomer bound to a streptavidin tetramer through a covalent bond between the Ig molecule and one of the streptavidin subunits. The conjugates were able to specifically target [111In]biocytin to HEY cells in vitro in the presence of human serum and ascitic fluid from ovarian cancer patients.
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