E Matteucci, L Pellegrini, C Uncini-Manganelli, R Navalesi, O Giampietro
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引用次数: 1
摘要
本文描述了一种灵敏、精确的尿乳酸脱氢酶(EC 1.1.1.27)、碱性磷酸酶(EC 3.1.3.1)和γ -谷氨酰转移酶(EC 2.3.2.2)的自动检测方法。为此，我们使用了BM/Hitachi System 704模型和用于勃林格曼海姆公司血清酶自动分析的试剂。然而，自动分析仪记录的酶化学参数表和分光光度校准表被重新编程，以满足尿液采用的要求，并优化这种不寻常介质中的酶分析。
Automated measurement of lactate dehydrogenase, alkaline phosphatase and gamma-glutamyltransferase in urines: an alternative to the manual procedure.
A sensitive and precise automated assay of urinary lactate dehydrogenase (EC 1.1.1.27), alkaline phosphatase (EC 3.1.3.1) and gamma-glutamyltransferase (EC 2.3.2.2) is described. For this purpose, we used a BM/Hitachi System 704 model and reagents for automated analysis of serum enzymes from Boehringer Mannheim. However, the schedules of enzyme chemistry parameters recorded by the autoanalyzer and the spectrophotometric calibration are reprogrammed to meet requirements deriving from urine adoption and to optimize the enzyme assay in this unusual medium.
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