E Matteucci, L Pellegrini, C Uncini-Manganelli, M Cecere, M Saviozzi, O Giampietro
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引用次数: 8
摘要
我们结论性的研究结果尿酶稳定性在样品储存期间报告。在-70℃和-20℃条件下，我们立即测量了9名健康正常受试者晨尿中的丙氨酸氨基肽酶(AAP)和n -乙酰- β - d -氨基葡萄糖苷酶(NAG)，并在-70℃条件下进行了1年的储存。结果表明，AAP在-70℃条件下相当稳定(年底基础值的99.2%)。NAG在-70℃时部分保存(基本值的84.1%)，但在-20℃时显著降低(50.4%)。
More on effects of storage time and temperature on urinary enzymes: a 1-year study.
Results of our conclusive study on urinary enzyme stability during sample storage are reported. We measured alanine aminopeptidase (AAP) and N-acetyl-beta-D-glucosaminidase (NAG) in morning urines from 9 healthy normal subjects immediately after collection and throughout a 1-year storage at -70 and -20 degrees C. AAP proved to be quite stable at -70 degrees C (99.2% of the basal value at the end of the year). NAG is partially preserved (84.1% of the basal value) at -70 degrees C, but significantly decreased (50.4%) at -20 degrees C.
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