P.O. Zamora , J.A. Mercer-Smith , M.J. Marek , L.D. Schulte , B.A. Rhodes
{"title":"人IgG中铜和锝结合位点的相似性","authors":"P.O. Zamora , J.A. Mercer-Smith , M.J. Marek , L.D. Schulte , B.A. Rhodes","doi":"10.1016/0883-2897(92)90142-L","DOIUrl":null,"url":null,"abstract":"<div><p>Kits for direct labeling of IgG with <sup>99m</sup>Tc were used without modification for the preparation of [<sup>67</sup>Cu]IgG. The IgG was pre-treated to generate thiolate groups which would bind <sup>67</sup>Cu. The direct labeling of reduced IgG with <sup>67</sup>Cu was highly efficient, resulting in approx. 95% <sup>67</sup>Cu binding. Non-reduced IgG (negative control) had labeling efficiencies of less than 10%. IgG pre-exposed to Cu(II) had reduced amounts of <sup>99m</sup>Tc bound to it. The results demonstrate a direct relationship between copper- and <sup>99m</sup>Tc-binding sites in IgG.</p></div>","PeriodicalId":14328,"journal":{"name":"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology","volume":"19 7","pages":"Pages 797-802"},"PeriodicalIF":0.0000,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0883-2897(92)90142-L","citationCount":"5","resultStr":"{\"title\":\"Similarity of copper and technetium binding sites in human IgG\",\"authors\":\"P.O. Zamora , J.A. Mercer-Smith , M.J. Marek , L.D. Schulte , B.A. Rhodes\",\"doi\":\"10.1016/0883-2897(92)90142-L\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Kits for direct labeling of IgG with <sup>99m</sup>Tc were used without modification for the preparation of [<sup>67</sup>Cu]IgG. The IgG was pre-treated to generate thiolate groups which would bind <sup>67</sup>Cu. The direct labeling of reduced IgG with <sup>67</sup>Cu was highly efficient, resulting in approx. 95% <sup>67</sup>Cu binding. Non-reduced IgG (negative control) had labeling efficiencies of less than 10%. IgG pre-exposed to Cu(II) had reduced amounts of <sup>99m</sup>Tc bound to it. The results demonstrate a direct relationship between copper- and <sup>99m</sup>Tc-binding sites in IgG.</p></div>\",\"PeriodicalId\":14328,\"journal\":{\"name\":\"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology\",\"volume\":\"19 7\",\"pages\":\"Pages 797-802\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0883-2897(92)90142-L\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/088328979290142L\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/088328979290142L","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Similarity of copper and technetium binding sites in human IgG
Kits for direct labeling of IgG with 99mTc were used without modification for the preparation of [67Cu]IgG. The IgG was pre-treated to generate thiolate groups which would bind 67Cu. The direct labeling of reduced IgG with 67Cu was highly efficient, resulting in approx. 95% 67Cu binding. Non-reduced IgG (negative control) had labeling efficiencies of less than 10%. IgG pre-exposed to Cu(II) had reduced amounts of 99mTc bound to it. The results demonstrate a direct relationship between copper- and 99mTc-binding sites in IgG.
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