{"title":"AF291和AE391单链抗体通过Western blotting识别HA标签","authors":"Z. Keszei, D. Picard","doi":"10.24450/JOURNALS/ABREP.2019.E25","DOIUrl":null,"url":null,"abstract":"The recombinant antibodies AF291and AE391 against the standard HA tag detect an HA-tagged protein exogenously expressed in human cells by Western blotting. However, similarly to one of the original monoclonal antibodies, they also recognize a number of unrelated endogenous proteins.","PeriodicalId":401643,"journal":{"name":"Antibody Reports","volume":"13 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"AF291 and AE391 single-chain antibodies recognize the HA tag by Western blotting\",\"authors\":\"Z. Keszei, D. Picard\",\"doi\":\"10.24450/JOURNALS/ABREP.2019.E25\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The recombinant antibodies AF291and AE391 against the standard HA tag detect an HA-tagged protein exogenously expressed in human cells by Western blotting. However, similarly to one of the original monoclonal antibodies, they also recognize a number of unrelated endogenous proteins.\",\"PeriodicalId\":401643,\"journal\":{\"name\":\"Antibody Reports\",\"volume\":\"13 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antibody Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.24450/JOURNALS/ABREP.2019.E25\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antibody Reports","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.24450/JOURNALS/ABREP.2019.E25","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
AF291 and AE391 single-chain antibodies recognize the HA tag by Western blotting
The recombinant antibodies AF291and AE391 against the standard HA tag detect an HA-tagged protein exogenously expressed in human cells by Western blotting. However, similarly to one of the original monoclonal antibodies, they also recognize a number of unrelated endogenous proteins.
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