用放射法检测病理材料中的分枝杆菌

M Slosárek, J Janota
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引用次数: 0

摘要

采用BACTEC放射法和标准培养技术同时检测96例疑似或确诊肺结核住院患者的病理标本。阳性样本总数为30份,仅BACTEC法阳性4份,仅培养法阳性10份。评估的差异无统计学意义。BACTEC法平均阳性时间为20.4天,培养28.8天。该栽培方法的高产是由多种因素引起的。BACTEC系统接种量为0.5 ml, 1个培养瓶;培养接种量为1.0 ml, 1个样品4根试管。从5个样本中,仅培养阳性,42-63天后仅获得零星菌落,并且在所有情况下仅在四种培养基中的一种培养基上获得。
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[Detection of mycobacteria in pathologic material using the radiometric method].

For concurrent detection of mycobacteria by the radiometric method BACTEC and the standard cultivation technique 96 samples of pathological material from patients hospitalized with suspected or confirmed tuberculosis was used. The total number of positive samples was 30, using the BACTEC method only, four samples and using the cultivation method only, 10 samples. The assessed difference was not statistically significant. The mean period of positivity with the BACTEC method was 20.4 days, in cultivations 28.8 days. The higher yield of the cultivation method could be caused by various factors. For the BACTEC system inocula of 0.5 ml were used and one cultivation bottle, for the cultivation inocula 1.0 ml and four test tubes for one sample were used. From five samples, positive only on cultivation, only sporadic colonies were obtained after 42-63 days cultivation and in all instances only on one of four media.

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