M Okabe, X Ying, M Nagira, M Ikawa, Y Kohama, T Mimura, K Tanaka
{"title":"顶体反应的精子特异性抗原与CD46的同源性。","authors":"M Okabe, X Ying, M Nagira, M Ikawa, Y Kohama, T Mimura, K Tanaka","doi":"10.1248/bpb1978.15.455","DOIUrl":null,"url":null,"abstract":"<p><p>MH61 is an acrosome-reacted sperm-specific monoclonal antibody. The antibody-coated beads effectively analyze the acrosomal status of human sperm. However, the nature of the antigen was not known. The antigen was purified by immunoprecipitation and SDS-PAGE followed by blotting on PVDF paper. The N-terminal sequence of the antigen was analyzed by an automated protein sequencer. An exactly matching sequence was found in CD46, that is also known as a membrane cofactor protein.</p>","PeriodicalId":16743,"journal":{"name":"Journal of pharmacobio-dynamics","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1992-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1248/bpb1978.15.455","citationCount":"29","resultStr":"{\"title\":\"Homology of an acrosome-reacted sperm-specific antigen to CD46.\",\"authors\":\"M Okabe, X Ying, M Nagira, M Ikawa, Y Kohama, T Mimura, K Tanaka\",\"doi\":\"10.1248/bpb1978.15.455\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>MH61 is an acrosome-reacted sperm-specific monoclonal antibody. The antibody-coated beads effectively analyze the acrosomal status of human sperm. However, the nature of the antigen was not known. The antigen was purified by immunoprecipitation and SDS-PAGE followed by blotting on PVDF paper. The N-terminal sequence of the antigen was analyzed by an automated protein sequencer. An exactly matching sequence was found in CD46, that is also known as a membrane cofactor protein.</p>\",\"PeriodicalId\":16743,\"journal\":{\"name\":\"Journal of pharmacobio-dynamics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1248/bpb1978.15.455\",\"citationCount\":\"29\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of pharmacobio-dynamics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1248/bpb1978.15.455\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of pharmacobio-dynamics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1248/bpb1978.15.455","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Homology of an acrosome-reacted sperm-specific antigen to CD46.
MH61 is an acrosome-reacted sperm-specific monoclonal antibody. The antibody-coated beads effectively analyze the acrosomal status of human sperm. However, the nature of the antigen was not known. The antigen was purified by immunoprecipitation and SDS-PAGE followed by blotting on PVDF paper. The N-terminal sequence of the antigen was analyzed by an automated protein sequencer. An exactly matching sequence was found in CD46, that is also known as a membrane cofactor protein.
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