粉煤灰微滤陶瓷膜分离蛋白质的研究

Vandana Gupta, J. Anandkumar
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引用次数: 4

摘要

本研究采用陶瓷微滤膜分离牛血清白蛋白(BSA)。采用掺有不同比例(2 ~ 8 wt%)的膨润土和掺有不同比例(20 wt%)的无机添加剂的粉煤灰制备陶瓷膜。采用扫描电子显微镜、x射线衍射分析、机械化学稳定性、孔隙率和纯水通量对合成的陶瓷膜进行了表征。陶瓷膜的力学稳定性和化学稳定性随饱泥含量的增加而提高。填充量为8% (C4)的陶瓷膜的最大抗折强度为20 MPa。C4膜孔隙率最低,为29.9%,渗透率0.397 L m-2h-1kPa-1,吸水能力20.15%,平均孔半径0.428 μm。研究了C4膜在进料浓度(200 ~ 1000 mg/L)、进料pH(2 ~ 10)、施加压力(68 ~ 482 kPa)等不同操作条件下对BSA的过滤效果。在最佳条件(208 kPa,自然pH, 200 mg/L浓度)下,BSA的最大去除率为82%,通量为81 L m-2 h-1。本研究结果表明,合成膜可作为蛋白质排斥应用的高效微滤膜。
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Protein Separation Using Fly-ash Microfiltration Ceramic Membrane
In this study, separation of protein (bovine serum albumin (BSA)) was carried out by ceramic microfiltration membranes. Ceramic membranes were fabricated by using fly-ash with different proportion (2-8 wt%) of fuller clay and fraction (20 wt%) of inorganic additives. Synthesized ceramic membranes were characterized using scanning electron microscope, X-ray diffraction analysis, mechanical-chemical stability, porosity and pure water flux. It was observed that the mechanical and chemical stability of ceramic membrane increases with increase in fuller clay’s content. Ceramic membrane with 8% fuller clay (C4) exhibited maximum flexural strength of 20 MPa. C4 membrane also shows least porosity of 29.9%, permeability of 0.397 L m-2h-1kPa-1, 20.15% water uptake capacity and 0.428 μm average pore radius. The BSA rejection efficiency of C4 membrane was studied for different operating parameter such as feed concentration (200-1000 mg/L), feed pH (2-10) and applied pressure (68-482 kPa). Maximum BSA rejection (82%) and flux (81 L m-2 h-1) has been observed at optimized condition (208 kPa, natural pH and 200 mg/L concentration). The results obtained in this work indicate that synthesized membrane could be used as proficient microfiltration membrane for protein rejection applications.
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