肿瘤启动子诱导晶状体混浊和H2O2升高。

Lens and eye toxicity research Pub Date : 1992-01-01

J J Ye, K Frenkel, J A Zadunaisky

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引用次数: 0

摘要

用10(-7)M的TPA (12- o - tetradecanoylphorbol13 -acetate, TPA)孵育全牛晶状体，24 h内晶状体不透明。用10(-7)M的TPA或2.5 mM的葡萄糖/20 μ M的葡萄糖氧化酶处理后，全牛晶状体中过氧化氢(H2O2)浓度提高4倍。晶状体混浊和H2O2升高与TPA剂量有关。用抗肿瘤促进剂EGCG(表没食子儿茶素没食子酸酯)或Sarp A (Sarp酚A)对晶状体进行预孵育，可阻止tpa介导的混浊过程，抑制H2O2升高。

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Lens opacification and H2O2 elevation induced by a tumor promoter.

Incubation of whole bovine lens with 10(-7) M 12-O-tetradecanoylphorbol-13-acetate (TPA) led to the lens opacity within 24 h. The hydrogen peroxide (H2O2) concentration in the whole lens was elevated 4 fold after treatment with either 10(-7) M TPA or 2.5 mM glucose/20 microM glucose oxidase. The lens opacification and H2O2 elevation were TPA dose-dependent. Preincubation of the lens with anti-tumor promoting agents EGCG (epigallocatechin gallate) or Sarp A (sarcophytol A) stopped the TPA-mediated opacification process and suppressed H2O2 elevation.

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Lens and eye toxicity research

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