Feng-Chieh Li, Chun-Chin Wang, Sung-Jan Lin, S. Jee, C. Dong
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Dorsal skinfold titanium chamber for noninvasive imaging in nude mice using multiphoton and harmonic generation microscopy
For many years, the dorsal skinfold chamber has been used for intravital observation in animal models. We have developed a chamber built using medical-grade titanium. Our design allows long-term, high resolution, and large-area observation in nude mice in vivo. Combined with two-photon fluorescence and second-harmonic generation microscopy, our chamber can be used to obtain qualitative and quantitative information for in vivo analysis of diseases such as tumors.
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