{"title":"过氧化氢在反胶束中氧化鲁米诺和异米诺皮质醇偶联物的化学发光增强。","authors":"D I Metelitza, A N Eryomin, V A Shibaev","doi":"10.1002/bio.1170070104","DOIUrl":null,"url":null,"abstract":"<p><p>The chemiluminescent oxidation of luminol and an isoluminol cortisol conjugate (ABI-COR) by hydrogen peroxide has been studied in cetyltrimethylammonium bromide (CTAB) reversed micelles in octane-chloroform (1:1). The maximum chemiluminescence intensity of both compounds is dependent on the initial concentrations of the H2O2 and substrates, the pH value of the micelle polar phase and the H2O/CTAB ratio. The optimum pH ranged from 8.5 to 9.5. Under comparable conditions, the chemiluminescence intensity for luminol was 15-fold higher than for the ABI-COR conjugate. A mechanism of oxidation of the substrates in reversed micelles is proposed and the possible mechanisms of inhibition by the substrate and oxidant is discussed.</p>","PeriodicalId":15068,"journal":{"name":"Journal of bioluminescence and chemiluminescence","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/bio.1170070104","citationCount":"7","resultStr":"{\"title\":\"Enhanced chemiluminescence in the oxidation of luminol and an isoluminol cortisol conjugate by hydrogen peroxide in reversed micelles.\",\"authors\":\"D I Metelitza, A N Eryomin, V A Shibaev\",\"doi\":\"10.1002/bio.1170070104\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The chemiluminescent oxidation of luminol and an isoluminol cortisol conjugate (ABI-COR) by hydrogen peroxide has been studied in cetyltrimethylammonium bromide (CTAB) reversed micelles in octane-chloroform (1:1). The maximum chemiluminescence intensity of both compounds is dependent on the initial concentrations of the H2O2 and substrates, the pH value of the micelle polar phase and the H2O/CTAB ratio. The optimum pH ranged from 8.5 to 9.5. Under comparable conditions, the chemiluminescence intensity for luminol was 15-fold higher than for the ABI-COR conjugate. A mechanism of oxidation of the substrates in reversed micelles is proposed and the possible mechanisms of inhibition by the substrate and oxidant is discussed.</p>\",\"PeriodicalId\":15068,\"journal\":{\"name\":\"Journal of bioluminescence and chemiluminescence\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/bio.1170070104\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of bioluminescence and chemiluminescence\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/bio.1170070104\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of bioluminescence and chemiluminescence","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/bio.1170070104","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Enhanced chemiluminescence in the oxidation of luminol and an isoluminol cortisol conjugate by hydrogen peroxide in reversed micelles.
The chemiluminescent oxidation of luminol and an isoluminol cortisol conjugate (ABI-COR) by hydrogen peroxide has been studied in cetyltrimethylammonium bromide (CTAB) reversed micelles in octane-chloroform (1:1). The maximum chemiluminescence intensity of both compounds is dependent on the initial concentrations of the H2O2 and substrates, the pH value of the micelle polar phase and the H2O/CTAB ratio. The optimum pH ranged from 8.5 to 9.5. Under comparable conditions, the chemiluminescence intensity for luminol was 15-fold higher than for the ABI-COR conjugate. A mechanism of oxidation of the substrates in reversed micelles is proposed and the possible mechanisms of inhibition by the substrate and oxidant is discussed.
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