杨梅素诱导人肝癌HepG2细胞凋亡和G2/M细胞周期阻滞

Xiu-juan Zhang, Qing-ling Huang, Yu-bin Ji
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引用次数: 0

摘要

本文研究杨梅素(myricetin, MYR)对人肝癌HepG2细胞凋亡的诱导作用。用不同剂量杨梅素处理人肝癌HepG2细胞72 h,用SRB法评价细胞毒性,用细胞化学染色(Hoechst 33258)和透射电镜(TEM)观察细胞凋亡相关形态学改变。流式细胞术测定细胞周期和凋亡率。
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Induction of apoptosis and G2/M cell cycle arrest by myricetin in human hepatoma HepG2 cells
The article is about induction of apoptosis in human hepatoma HepG2 cells by myricetin (MYR). The human hepatoma HepG2 cells was treated with different doses myricetin for 72 h. Cytotoxicity was evaluated by SRB assay, apoptosis-related alterations in morphology ascertained by cytochemical staining(Hoechst 33258) and transmission electron microscopy(TEM). The cell cycle and apoptotic rate were quantified by flow cytometry (FCM).
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