N. A. Yudhaswara, A. Prijanti, M. Sadikin
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摘要

背景:硫辛酸是一种存在于细胞内和细胞外的物质,是丙酮酸脱氢酶的辅酶,也是一种解毒剂、螯合剂和抗氧化剂。需要测量硫辛酸来确定硫辛酸的量,以发挥其作为辅酶或抗氧化剂的功能。此外,这种测量需要一个特殊的工具,如高效液相色谱(HPLC)和一个在农村或简单实验室可用的过程。目的:建立一种简便易行的分光光度计测定血浆等体液中硫辛酸的方法。方法:采用紫外甲醇分光光度法和可见PdCl2分光光度法测定硫辛酸,并与高效液相色谱法进行比较,该方法在药物或制剂的测定中是有效可靠的。结果:PdCl2法测定血浆和白细胞中硫辛酸,结果可重复性好、可靠、有效,准确度、精密度高,与高效液相色谱法测定硫辛酸无差异,p=0.99。而紫外甲醇与高效液相色谱的差异p =0.0001或无效。结论:PdCl2可见法测定硫辛酸可用于测定血浆中硫辛酸(LA)和DHLA的含量,与高效液相色谱法测定结果相当。
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PALLADIUM (II) CHLORIDE (PdCl2) SPECTROPHOTOMETRY TO DETERMINE LIPOIC ACID CONCENTRATION IN PLASMA AND LEUKOCYTES
Background: Lipoic acid is a substance contained in intra and extracellular that act as a coenzyme of Pyruvate Dehydrogenase, also as an antidote, chelating agent and antioxidant. Measurement of lipoic acid is needed to determine the amount of lipoic acid that performs its functions either as a coenzyme or an antioxidant. Besides, this measurement requires a special tool such as High Performance Liquid Chromatography (HPLC) and a process that is available in rural or simple laboratories.  Objective: A common and easy tool such as a spectrophotometer was conducted and could expected to be a tool of lipoic acid determination in body fluid such as plasma.Methods: Measurement of lipoic acid using spectrophotometry with UV methanol and visible PdCl2 has been tested and compared to HPLC measurement that was valid and reliable in drug measurement or pharmaceutical preparations.Results: Determination of lipoic acid in plasma and leukocytes using PdCl2 produced replicable, reliability and valid result, with high accuracy, precision and was not different from lipoic acid measurement using HPLC, p=0.99. While UV methanol was different compare to HPLC p =0.0001 or was not valid.Conclusion: The measurement of lipoic acid using PdCl2 visible method can be applied to determine the levels of lipoic acid (LA) and DHLA in plasma and equal to HPLC result.
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