玉米Lc调控基因对转基因烟草和矮牵牛花色的影响

Yang Chi-Chun, Yu Jing-juan, Z. Qian, Zhu Dengyun, Ao Guang-ming
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引用次数: 5

摘要

从油菜(Brassica napus)基因组中扩增出XY355启动子,该启动子是一个花瓣特异性启动子。构建了含XY355启动子和玉米Lc调控基因的植物表达载体pXY60。采用农杆菌介导法将该载体分别转染烟草(Nicotiana tabacum)和矮牵牛(petunia hybrida)。对这些再生植株的PCR分析结果表明,Lc基因分别被整合到转基因烟草和矮牵牛的基因组中。转基因植物的花色发生了变化,转基因烟草的花色由浅红色变为红色,转基因矮牵牛的花色由白色变为浅紫色。
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Influence of maize Lc regulatory gene on flower colour of transgenic tobacco and petunia
XY355 promoter, a petal-specific promoter, was amplified from the genome of rape(Brassica napus ) by PCR. The plant expression vector pXY60,which contained XY355 promoter and the maize Lc regulatory gene, was constructed. The vector was respectively transfered into tobacco(Nicotiana tabacum)and petunia(Petunia hybrida )by Agrobacterium tumefaciens-mediated method. The results of PCR analysis among these regenerated plants indicated that Lc gene was integrated into the genomes of transgenic tobacco and petunia, respectively. The flower color of transgenic plants had been changed, the color of transgenic tobacco was changed from light red to red and that of transgenic petunia was changed from white to light purple.
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