{"title":"Induksi Kalus Kentang Asal Desa Dombu (Solanum tuberosum L.) Dengan Zpt Indole-3-Acetic Acid (IAA)","authors":"Lyly Zulraufianti, Asri Pirade Paserang","doi":"10.22487/25411969.2019.v8.i2.13546","DOIUrl":null,"url":null,"abstract":"The research of callus induction in potato (S. tuberosum L.) from Dombu Village with indole-3-acetic-acid (IAA) was conducted from Januari to April 2019 in Laboratory of Tissue Culture, Biology Department, Faculty of Mathematics and Natural Science, Tadulako University. The research was aimed to determine of concentration IAA of the best to induce callus. This study was designed based on Completely Randomized Design (CRD) with 5 treatments, 3 replications and 3 explants on each unit. The treatments were P1= MS + 0 ppm, P2= MS + 0.5 ppm, P3= MS + 1.0 ppm, P4= MS + 2.0 ppm, P5= MS + 3.0 ppm. The results showed that the best and the efficient treatments for induction of callus treatment was P4= MS + 2.0 ppm with to induce callus up to 100%, the callus colour was yellow to brown and intermediate texture.","PeriodicalId":399499,"journal":{"name":"Natural Science: Journal of Science and Technology","volume":"36 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Science: Journal of Science and Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22487/25411969.2019.v8.i2.13546","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
2019年1 - 4月,在大田大学数学与自然科学学院生物系组织培养实验室对东布村马铃薯(S. tuberosum L.)进行了吲哚-3-乙酸(IAA)诱导愈伤组织的研究。研究旨在确定诱导愈伤组织的最佳IAA浓度。本试验采用完全随机设计(CRD), 5个处理,3个重复,每个单元3个外植体。处理为P1= MS + 0 ppm、P2= MS + 0.5 ppm、P3= MS + 1.0 ppm、P4= MS + 2.0 ppm、P5= MS + 3.0 ppm。结果表明,P4= MS + 2.0 ppm是诱导愈伤组织的最佳和有效处理,愈伤组织诱导率高达100%,愈伤组织颜色为黄至棕色,质地中等。
Induksi Kalus Kentang Asal Desa Dombu (Solanum tuberosum L.) Dengan Zpt Indole-3-Acetic Acid (IAA)
The research of callus induction in potato (S. tuberosum L.) from Dombu Village with indole-3-acetic-acid (IAA) was conducted from Januari to April 2019 in Laboratory of Tissue Culture, Biology Department, Faculty of Mathematics and Natural Science, Tadulako University. The research was aimed to determine of concentration IAA of the best to induce callus. This study was designed based on Completely Randomized Design (CRD) with 5 treatments, 3 replications and 3 explants on each unit. The treatments were P1= MS + 0 ppm, P2= MS + 0.5 ppm, P3= MS + 1.0 ppm, P4= MS + 2.0 ppm, P5= MS + 3.0 ppm. The results showed that the best and the efficient treatments for induction of callus treatment was P4= MS + 2.0 ppm with to induce callus up to 100%, the callus colour was yellow to brown and intermediate texture.