{"title":"有机共溶剂对热溶菌素合成和水解肽的不同影响。","authors":"D E Nevin, R J Beynon","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Thermolysin is an effective peptide synthetase in mixtures of buffer and water miscible solvents MeOH, EtOH and PrOH. Although the solvents are inhibitory, primarily through effects on kcat, synthetic reactions are much less affected than peptide hydrolysis. This may be of value in prevention of unwanted proteolytic cleavages, for example in protein semisynthesis.</p>","PeriodicalId":8948,"journal":{"name":"Biomedica biochimica acta","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Differential effects of organic co-solvents on peptide synthesis and hydrolysis by thermolysin.\",\"authors\":\"D E Nevin, R J Beynon\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Thermolysin is an effective peptide synthetase in mixtures of buffer and water miscible solvents MeOH, EtOH and PrOH. Although the solvents are inhibitory, primarily through effects on kcat, synthetic reactions are much less affected than peptide hydrolysis. This may be of value in prevention of unwanted proteolytic cleavages, for example in protein semisynthesis.</p>\",\"PeriodicalId\":8948,\"journal\":{\"name\":\"Biomedica biochimica acta\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedica biochimica acta\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedica biochimica acta","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Differential effects of organic co-solvents on peptide synthesis and hydrolysis by thermolysin.
Thermolysin is an effective peptide synthetase in mixtures of buffer and water miscible solvents MeOH, EtOH and PrOH. Although the solvents are inhibitory, primarily through effects on kcat, synthetic reactions are much less affected than peptide hydrolysis. This may be of value in prevention of unwanted proteolytic cleavages, for example in protein semisynthesis.