统计研究在克氏锥虫哥伦比亚菌株形态计量学特征中的应用。

J A De Diego, P Penin, F Mayer, J Delrey, C Gamallo
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引用次数: 0

摘要

经过形态学和组织病理学鉴定后,从哥伦比亚卡利(Cali)的一名患者身上分离的克氏锥虫Cali菌株也进行了形态计量学鉴定。为此,我们通过IBAS-1(图像基本分析系统)的平面测量,估计了NNN培养基中血液中每种进化形式的30个样本中的16个细胞参数。本形态计量学研究通过应用方差(ANOVA-1)完成统计研究。这使得有可能证明细长,宽和粗壮形式之间的大量显着差异,以及来自NNN介质的短和大的外生毛囊之间的差异。这些数据对克氏锥虫生物循环的某些特征分类以及诊断技术或治疗和预防实验的未来发展具有真正的意义。
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The application of the statistical study in the morphometric characterization of a Colombian strain of Trypanosoma cruzi.

After morphological and histopathological characterization, the Cali strain of T. cruzi isolated from a patient in Cali (Colombia), was also submitted to a morphometrical characterization. For this we estimated 16 cellular parameters in 30 samples of each evolutive form of T. cruzi in blood in NNN medium through planimetry by the IBAS-1 (Image Basic Analytic System). This morphometrical study was completed with a statistical one through the application of the variance (ANOVA-1). This made it possible to evidence a great number of significant differences between the slender, broad and stout forms and also between the short and large epimastigotes from the NNN medium. These data have a real interest for classifying some features of the biological cycle of T. cruzi as well as the future development of diagnosis techniques or therapeutical and prophylactic experiments.

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