椰子水对‘泰美’钙双色Cv微繁的影响

Raveena D Ratnayake, S. Peiris, Gayani Malwattage, C. N. Peiris
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摘要

Caladium双色(Aiton)喷口是一种极具吸引力的商业观赏植物,在园艺业中作为盆栽植物和园林绿化植物很受欢迎。这种有吸引力的植物通过微繁繁殖比常规繁殖有更多的好处。目前的研究描述了用椰子水作为补充的Caladium双色cv ' Thai Beauty '的体外增殖。叶片外植体的芽在分别添加0、0.5、1和1.5 mg/L(6-苄基氨基嘌呤)和不添加120 mL/L椰子水的Murashige和Skoog (MS)培养基上培养。结果表明,添加0.5和1.5 mg/L BAP的椰子水能促进钙芽增殖,平均分别为6.67和7.75个芽/枝,并能产生大量优质芽。培养基中仅椰子水也平均产生3.42个芽/芽。因此,目前的协议提供了一种直接的,具有成本效益的大规模繁殖方法,用于大规模的商业化培养钾。关键词:贝母;微体繁殖;椰子汁;6-benzylaminopurine
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Effects of Coconut Water on Micropropagation of Caladium Bicolour Cv ’Thai Beauty’
Caladium bicolour (Aiton) Vent. is an attractive commercial ornamental plant in the horticulture industry which is popular as potted plants for homescaping and as garden plants for landscaping. Propagation of this attractive plant through micropropagation has more benefits than conventional propagation. The current study describes an in vitro multiplication of Caladium bicolour cv ‘Thai Beauty’ with coconut water as a supplement. Shoots derived from leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1 and 1.5 mg/L (6- Benzylaminopurine) with and without 120 mL/L of coconut water. The results showed that coconut water (CW) with 0.5 and 1.5 mg/L BAP in the medium increased caladium shoot multiplication having an average of 6.67 and 7.75 shoots /shoot, respectively, with a large number of high-quality shoots. Coconut water alone in the medium also produced average of 3.42 shoots/ shoot. Hence, the current protocol provides a direct, cost-effective mass propagation method for large-scale commercial cultivation of caladium. Keywords: Caladium; Micropropagation; Coconut water; 6-benzylaminopurine
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