荧光比率法和荧光寿命(FLIM)成像:单分子转子双模式成像细胞粘度

Zhigang Yang, Jiangli Fan, Xiaojun Peng
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引用次数: 2

摘要

细胞内黏度强烈影响物质和信号的运输、生物大分子之间的相互作用以及活性代谢物在活细胞(如ROS和RNS)中的扩散。细胞内黏度变化与许多疾病和病理有关。因此,在细胞水平上研究微粘度具有重要意义。荧光分子转子是近年来发展起来的用于测定环境粘度的传感器。由于活细胞的复杂性,为保证高可靠性和准确性,进行多模态粘度测定非常重要。报道了第一个能够双模式荧光成像(比率成像和荧光寿命成像)细胞内粘度的分子转子(RY)。[1]
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Fluorescence ratiometry and fluorescence lifetime (FLIM) imaging: Dual mode imaging cellular viscosity by a single molecular rotor
Intracellular viscosity strongly influences transportation of mass and signal, interactions between the biomacromolecules, and diffusion of reactive metabolites in live cells such as ROS and RNS. Intracellular viscosity changes relate to a number of diseases and pathologies. So it is meaningful to investigate the microviscosity at cellular level. Fluorescent molecular rotors are recently developed sensors used to determine the environmental viscosity. Due to the complexity of live cells, it is important to carry out the viscosity determinations in multimode for high reliability and accuracy. The first molecular rotor (RY) capable of dual mode fluorescence imaging (ratiometry imaging and fluorescence lifetime imaging) of intracellular viscosity is reported.[1]
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