G Bánhegyi, T Garzó, G Mészáros, F Antoni, J Mandl
{"title":"小鼠肝脏中胆红素和吗啡糖醛酸化不受环amp介导机制的抑制。","authors":"G Bánhegyi, T Garzó, G Mészáros, F Antoni, J Mandl","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Several reactions of the Ist and IInd phase of biotransformation have been reported to be under a negative cyclic-AMP dependent control. Conjugation of bilirubin and glucuronidation of morphine were investigated in isolated mouse hepatocytes and in mouse hepatocyte microsomes, respectively. N6,O2-dibutyryl cyclic-AMP did not inhibit conjugation of bilirubin in isolated hepatocytes. ATP and the dissociated catalytic subunit of the cyclic-AMP dependent protein kinase did not influence the glucuronidation of morphine, while inhibited the formation of p-nitrophenol glucuronide in microsomes prepared from isolated mouse hepatocytes.</p>","PeriodicalId":77479,"journal":{"name":"Acta biochimica et biophysica Hungarica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bilirubin and morphine glucuronidation is not inhibited by a cyclic-AMP mediated mechanism in murine liver.\",\"authors\":\"G Bánhegyi, T Garzó, G Mészáros, F Antoni, J Mandl\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Several reactions of the Ist and IInd phase of biotransformation have been reported to be under a negative cyclic-AMP dependent control. Conjugation of bilirubin and glucuronidation of morphine were investigated in isolated mouse hepatocytes and in mouse hepatocyte microsomes, respectively. N6,O2-dibutyryl cyclic-AMP did not inhibit conjugation of bilirubin in isolated hepatocytes. ATP and the dissociated catalytic subunit of the cyclic-AMP dependent protein kinase did not influence the glucuronidation of morphine, while inhibited the formation of p-nitrophenol glucuronide in microsomes prepared from isolated mouse hepatocytes.</p>\",\"PeriodicalId\":77479,\"journal\":{\"name\":\"Acta biochimica et biophysica Hungarica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta biochimica et biophysica Hungarica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta biochimica et biophysica Hungarica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Bilirubin and morphine glucuronidation is not inhibited by a cyclic-AMP mediated mechanism in murine liver.
Several reactions of the Ist and IInd phase of biotransformation have been reported to be under a negative cyclic-AMP dependent control. Conjugation of bilirubin and glucuronidation of morphine were investigated in isolated mouse hepatocytes and in mouse hepatocyte microsomes, respectively. N6,O2-dibutyryl cyclic-AMP did not inhibit conjugation of bilirubin in isolated hepatocytes. ATP and the dissociated catalytic subunit of the cyclic-AMP dependent protein kinase did not influence the glucuronidation of morphine, while inhibited the formation of p-nitrophenol glucuronide in microsomes prepared from isolated mouse hepatocytes.