{"title":"atp敏感的K(+)通道损耗依赖于Mg2+。","authors":"R Z Kozlowski, M L Ashford","doi":"10.1098/rspb.1990.0044","DOIUrl":null,"url":null,"abstract":"<p><p>ATP-sensitive K(+)-channel currents were recorded from isolated membrane patches and voltage-clamped CRI-G1 insulin-secreting cells. Internal Mg2+ ions inhibited ATP-K+ channels by a voltage-dependent block of the channel current and decrease of open-state probability. The run-down of ATP-K+ channel activity was also shown to be [Mg2+]i dependent, being almost abolished in Mg2(+)-free conditions. Substitution of Mn2+ for Mg2+ did not prevent run-down, nor did the presence of phosphate-donating nucleotides, a protease or phosphatase inhibitor or replacement of Cl- by gluconate.</p>","PeriodicalId":54561,"journal":{"name":"Proceedings of the Royal Society of London Series B-Containing Papers of Abiological Character","volume":"240 1298","pages":"397-410"},"PeriodicalIF":0.0000,"publicationDate":"1990-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1098/rspb.1990.0044","citationCount":"59","resultStr":"{\"title\":\"ATP-sensitive K(+)-channel run-down is Mg2+ dependent.\",\"authors\":\"R Z Kozlowski, M L Ashford\",\"doi\":\"10.1098/rspb.1990.0044\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>ATP-sensitive K(+)-channel currents were recorded from isolated membrane patches and voltage-clamped CRI-G1 insulin-secreting cells. Internal Mg2+ ions inhibited ATP-K+ channels by a voltage-dependent block of the channel current and decrease of open-state probability. The run-down of ATP-K+ channel activity was also shown to be [Mg2+]i dependent, being almost abolished in Mg2(+)-free conditions. Substitution of Mn2+ for Mg2+ did not prevent run-down, nor did the presence of phosphate-donating nucleotides, a protease or phosphatase inhibitor or replacement of Cl- by gluconate.</p>\",\"PeriodicalId\":54561,\"journal\":{\"name\":\"Proceedings of the Royal Society of London Series B-Containing Papers of Abiological Character\",\"volume\":\"240 1298\",\"pages\":\"397-410\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-06-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1098/rspb.1990.0044\",\"citationCount\":\"59\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the Royal Society of London Series B-Containing Papers of Abiological Character\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1098/rspb.1990.0044\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Royal Society of London Series B-Containing Papers of Abiological Character","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1098/rspb.1990.0044","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
ATP-sensitive K(+)-channel run-down is Mg2+ dependent.
ATP-sensitive K(+)-channel currents were recorded from isolated membrane patches and voltage-clamped CRI-G1 insulin-secreting cells. Internal Mg2+ ions inhibited ATP-K+ channels by a voltage-dependent block of the channel current and decrease of open-state probability. The run-down of ATP-K+ channel activity was also shown to be [Mg2+]i dependent, being almost abolished in Mg2(+)-free conditions. Substitution of Mn2+ for Mg2+ did not prevent run-down, nor did the presence of phosphate-donating nucleotides, a protease or phosphatase inhibitor or replacement of Cl- by gluconate.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
