形态分析和DNA条形码在万丹苔原岛瓷蟹种类鉴定中的应用

T. Triandiza, Hawis Maddupa
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摘要

海洋隐种现象经常出现在生态系统发生变化的地方,如死珊瑚栖息地。死珊瑚可以作为海洋动物的栖息地,如贝壳科甲壳类动物。本研究旨在利用形态分析和DNA条形码技术对万丹省屯台岛瓷蟹的种类进行鉴定和澄清。形态学分析结果表明,十足类标本属于蟹科蟹蟹属,其特殊形态特征是甲壳尺寸比外型长,触角长且可移动,前喙呈锯齿状,螯爪扁平,臂大小不等。基于COI的分子条形码分析,样品的相似性为94%,鉴定为Anomura sp.。结果的差异被怀疑是由于基因库中缺乏合适的遗传数据。
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Application of Morphological Analysis and DNA Barcode in Determination of The Porcelain Crab Species (Pisidia sp.) from The Tunda Island, Banten
The phenomenon of marine cryptic species is frequently found in altered ecosystem such as dead corals habitat. Dead corals as habitat can be used by marine fauna like crustacean from family of porcellanidae. This study was aimed to identify and clarify the species of porcelain crab from Tundai sland, Banten province by applying the morphological analysis and DNA barcoding technique. The result of morphological analysis showed that decapod samples were crabs of the genus Pisidia, family of Porcellanidae with specific morphological characteristics were the ratio of carapace size is longer than tbroad, have long antennae which can be moved, serrated anterior rostrum, chelae rather flattish and unequal size of arms. While based on molecular barcode analysis by using COI, samples were identified as Anomura sp. with 94% of similarities. The difference of the result was suspected due to the lack of suitable genetic data on genbank.
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