{"title":"药用植物水芹叶片愈伤组织再生植株的研究","authors":"Prakasha, K. Rave, Kabballi Balakrishn, G. Lokesh","doi":"10.3923/AJBKR.2012.143.146","DOIUrl":null,"url":null,"abstract":"Plantlets regeneration through callus has been achieved in medicinal plant Aerva lanata of Amaranthaceae. Callus obtained by culturing young leaf disks on Murashige and Skoog's (MS) medium with 1.0 mg L-1 2, 4.D (dichlorophenoxyacetic acid) was subcultured on MS medium contg. 2.0 mg L-1 6, Benzyl Amino Purine (BAP), produced 30-40 multiple shoots. Rooting was achieved on half or full strength MS medium with 0.5-0.1 mg L-1 Naphthalene Acetic Acid (NAA) in 14 days. Regenerated plantlets were acclimatized and transferred to soil were showed normal morphol. characteristics. Survival of transplants was 90-95% under green house condition. [on SciFinder(R)]","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Regeneration of Plantlets from Leaf Derived Callus of Aerva lanata, Juss: A Medicinal Plant\",\"authors\":\"Prakasha, K. Rave, Kabballi Balakrishn, G. Lokesh\",\"doi\":\"10.3923/AJBKR.2012.143.146\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Plantlets regeneration through callus has been achieved in medicinal plant Aerva lanata of Amaranthaceae. Callus obtained by culturing young leaf disks on Murashige and Skoog's (MS) medium with 1.0 mg L-1 2, 4.D (dichlorophenoxyacetic acid) was subcultured on MS medium contg. 2.0 mg L-1 6, Benzyl Amino Purine (BAP), produced 30-40 multiple shoots. Rooting was achieved on half or full strength MS medium with 0.5-0.1 mg L-1 Naphthalene Acetic Acid (NAA) in 14 days. Regenerated plantlets were acclimatized and transferred to soil were showed normal morphol. characteristics. Survival of transplants was 90-95% under green house condition. [on SciFinder(R)]\",\"PeriodicalId\":274901,\"journal\":{\"name\":\"Asian Journal of Biotechnology\",\"volume\":\"13 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Asian Journal of Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3923/AJBKR.2012.143.146\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asian Journal of Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3923/AJBKR.2012.143.146","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
摘要
苋菜科药用植物紫花苜蓿已经实现了愈伤组织再生。幼叶盘在含1.0 mg l - 1,2,4的Murashige和Skoog培养基上培养愈伤组织。二氯苯氧乙酸在MS培养基上传代培养。2.0 mg l - 16，苄基氨基嘌呤(BAP)，产生30-40个多枝。在含0.5 ~ 0.1 mg L-1萘乙酸(NAA)的半强或全强MS培养基上生根，培养时间为14 d。对再生植株进行驯化和移栽，使其形态恢复正常。特征。温室条件下移植物成活率为90-95%。(在SciFinder (R))
Regeneration of Plantlets from Leaf Derived Callus of Aerva lanata, Juss: A Medicinal Plant
Plantlets regeneration through callus has been achieved in medicinal plant Aerva lanata of Amaranthaceae. Callus obtained by culturing young leaf disks on Murashige and Skoog's (MS) medium with 1.0 mg L-1 2, 4.D (dichlorophenoxyacetic acid) was subcultured on MS medium contg. 2.0 mg L-1 6, Benzyl Amino Purine (BAP), produced 30-40 multiple shoots. Rooting was achieved on half or full strength MS medium with 0.5-0.1 mg L-1 Naphthalene Acetic Acid (NAA) in 14 days. Regenerated plantlets were acclimatized and transferred to soil were showed normal morphol. characteristics. Survival of transplants was 90-95% under green house condition. [on SciFinder(R)]
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
