[用小型计算机测定病毒浓度]。

T Smola, G Specht, H Liebermann
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引用次数: 0

摘要

本文描述了一种确定病毒浓度的物理方法,包括通过糖梯度对样品进行超离心,随后通过流动光度计对小管含量进行分析。在此之前,这样得到的曲线是用模板进行图形化评估的。用kc85 /1型小型计算机直接记录和处理消光时间曲线,大大提高了结果的准确性。减少了时间消耗,并且完全排除了读取和计算错误。重复测定结果证实,测定病毒浓度的准确度可达4%至7%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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[Determination of virus concentration using a minicomputer].

Described in this paper is a physical method by which to determine virus concentrations, including ultracentrifugation of a sample through a saccharose gradient and subsequent analysis of tubule content by means of a flow photometer. Here before, the curves thus obtained used to be graphically evaluated by means of stencil plates. Direct recording and processing of the extinction-time curve by means of a KC 85/1 minicomputer have substantially improved accuracy of results. Time consumption has been reduced, and reading as well as calculating errors have been entirely ruled out. Repetition of determinations confirmed that virus concentrations could be determined at an accuracy of four to seven percent.

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