植物细胞和组织培养的体外再生及其应用

M Jordán
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引用次数: 0

摘要

利用植物细胞的全能性和体外培养技术,可以诱导植物细胞进行形态发生,形成体细胞胚和大量克隆增殖;通过触发小孢子或花粉恢复单倍体植株,选择和固定单倍体表达的性状。来自不同物种的原生质体可以导致重组。我们在这里报告了在毛状体上所做的工作,从细胞中获得了体细胞胚;在烟叶花药中恢复植株的同时,引发了鸟李雄激素发生导致花粉愈伤组织。利用短毛桃和木瓜原生质体获得融合产物,形成愈伤组织和芽。
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[In vitro regeneration and applications using vegetable cell and tissue culture].

Plant cells by means of their totipotency and aided by in vitro culture techniques can be induced to perform morphogenesis leading to somatic embryoids and massive clonal multiplication; microspores or pollen can be triggered to recover haploid plants, then characters expressed via haploidy can be selected and fixed. Protoplasts from different species can lead to recombinations. We report here work done on Carica pubescens, where somatic embryoids were obtained from cells; in Prunus avium androgenesis leading to pollen calli was triggered, while plants were recovered from Nicotiana tabacum anthers. Fusion products were obtained using C. pubescens and C. papaya protoplasts, leading up to calli and shoots.

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The evolution of hexokinases. [Hermann Niemeyer Fernández (1918-1991) and science in Chile]. Society of Biology of Chile and the Associated Societies, 34th annual meeting. Puyehue, Chile, 27-30 November 1991. Abstracts. [Preparation and characterization of a monoclonal peroxidase-antiperoxidase complex]. [Neurochemical substrate of the behavioral pharmacology of ethanol].
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