{"title":"凝胶过滤色谱法纯化噬菌体DNA","authors":"Ana González, Jaime Gómez-Márquez","doi":"10.1016/0735-0651(90)90037-G","DOIUrl":null,"url":null,"abstract":"<div><p>Two fast and effective methods for high-scale purification of linear phage λ DNA and circular double-stranded M13 replicative form are presented. A substantial reduction of time is attained by avoiding the long-term CsCl gradient centrifugations and dialysis common to standard procedures. Biologically active DNA preparations, free of chromosomal DNA and RNA, are obtained by including a simple gel filtration chromatography as the last step of purification. Yields are comparable to those from previously described methods.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"7 1","pages":"Pages 2-4"},"PeriodicalIF":0.0000,"publicationDate":"1990-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(90)90037-G","citationCount":"5","resultStr":"{\"title\":\"Purification of bacteriophage DNA by gel filtration chromatography\",\"authors\":\"Ana González, Jaime Gómez-Márquez\",\"doi\":\"10.1016/0735-0651(90)90037-G\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Two fast and effective methods for high-scale purification of linear phage λ DNA and circular double-stranded M13 replicative form are presented. A substantial reduction of time is attained by avoiding the long-term CsCl gradient centrifugations and dialysis common to standard procedures. Biologically active DNA preparations, free of chromosomal DNA and RNA, are obtained by including a simple gel filtration chromatography as the last step of purification. Yields are comparable to those from previously described methods.</p></div>\",\"PeriodicalId\":77714,\"journal\":{\"name\":\"Gene analysis techniques\",\"volume\":\"7 1\",\"pages\":\"Pages 2-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0735-0651(90)90037-G\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene analysis techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/073506519090037G\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/073506519090037G","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Purification of bacteriophage DNA by gel filtration chromatography
Two fast and effective methods for high-scale purification of linear phage λ DNA and circular double-stranded M13 replicative form are presented. A substantial reduction of time is attained by avoiding the long-term CsCl gradient centrifugations and dialysis common to standard procedures. Biologically active DNA preparations, free of chromosomal DNA and RNA, are obtained by including a simple gel filtration chromatography as the last step of purification. Yields are comparable to those from previously described methods.
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