Wistar大鼠(Rattus norvegicus Berkenhout)咀嚼肌的产后发育。组织化学研究]。

Anatomischer Anzeiger Pub Date : 1990-01-01
R Kowalewski, M Miltzow
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引用次数: 0

摘要

采用酶组织化学方法对Wistar大鼠(Rattus norvegicus Berkenhout)下颌骨内收肌的肌肉活检组织进行了分析,以研究其产后发育(42 -126)。D产后)的肌纤维,特别是纤维的类型。本研究采用了以下方法:肌纤维腺苷三磷酸酶(atp酶)与不同的预培养,苏丹黑B(甘油三酯),周期性酸-希夫试剂(PAS)(糖原),Gomori修饰的三色染色。定量分析了不同区域肌肉切片的肌纤维类型组成和肌纤维大小。通过测量每种肌肉纤维的最小直径来估计纤维的大小。
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[Postnatal development of the masticatory muscles of the Wistar rat (Rattus norvegicus Berkenhout). A histochemical study].

Muscle biopsies of the mandible adductors of the Wistar rat (Rattus norvegicus Berkenhout) have been analyzed enzyme-histochemically for the investigation of the postnatal development (42.-126. d post partum) of their muscle fibers with special regards to the fiber types. The following methods have been used in this investigation: myofibrillar adenosine triphosphatase (ATP-ase) with different pre-incubations, Sudan black B (triglycerides), periodic acid-Schiff reagent (PAS) (glycogen), Gomori modified Trichrome staining. Quantitative analysis of muscle fibre-type composition and muscle fibre size was done from different regions of muscle sections. Estimation of the fiber size was carried out by measuring the minimum diameter of each type of muscle fibre.

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