紫苏与hirtella定位群体种间杂交qtl的检测

M. Lee, K. Oh, M. Kim, Sung Up Kim, Jung-In Kim, Eunyoung Oh, S. Pae, U. Yeo, Tae-Ho Kim, Jeong Hee Lee, Chan-Sik Jung, D. Kwak, and Yong Chul Kim
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引用次数: 3

摘要

柠檬酸假单胞菌与hirtella假单胞菌的种间杂交为紫苏基因组计划中连锁图谱的构建提供了理想的材料。在n=10时,两种染色体相同,杂交后代生长和结实率正常。杂交F2群体的表型呈正态分布,这证明了染色体在有性繁殖过程中具有很高的亲和力。F2植株迷迭香酸、木犀草素和芹菜素含量与四倍体紫苏品种分布范围相近。木犀草素与芹菜素正相关,相关系数为0.762。农艺性状和迷迭香酸、木犀草素、芹菜素含量共检测到21个qtl。尽管木犀草素与芹菜素呈正相关,但qtl定位位置不同。在第3染色体上定位紫色叶片颜色相关QTL, LOD为14.3,PVE为50.4%。在QTL附近检测到3个花青素生物合成转录因子样序列,即WD40重复样超家族蛋白、myb结构域蛋白43和碱性螺旋-环-螺旋(bHLH) dna结合超家族蛋白。
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Detection of QTLs in an Interspecific Cross between Perilla citriodora × P. hirtella Mapping Population
An interspecific cross between P. citriodora and P. hirtella constitutes ideal material for a linkage map construction in genome project of Perilla. The chromosomes of the species are same with n=10 and progenies of the cross are normal in growth and seed set. The phenotype of F2 population of the cross are normally distributed and this is a proof of high affinity of the chromosomes during their sexual reproduction. Rosmarinic acid, Luteolin and Apigenin contents of F2 plants were distributed in similar range with tetraploid perilla cultivars. Luteolin and Apigenin are positively correlated with correlation coefficient of 0.762. 21 QTLs were detected in agronomic traits and Rosmarinic acid, Luteolin and Apigenin contents. Even though Luteolin and Apigenin are positively correlated, QTLs were located in different position. Purple leaf color related QTL was mapped in Chromosome 3 with LOD of 14.3, PVE of 50.4%. Three anthocyanin biosynthesis transcription factor like sequences, WD40 repeat-like superfamily protein, myb domain protein 43 and basic helix-loop-helix (bHLH) DNA-binding superfamily protein, were detected near from the QTL.
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