[收集大鼠胰液的技术]。

A Königsrainer, C Habringer, R Krausler, G Klima, R Margreiter
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引用次数: 0

摘要

监测外分泌功能和胰液细胞学已被证明是检测胰腺异体移植排斥反应的可靠方法。为了证实这些临床发现并确定进一步的参数,仍需要进行实验工作。选用大鼠进行实验,并建立了胰液获取技术。在5只动物中,使用了完全植入式封闭储层导管系统。为了防止细菌污染,有必要在水库中安装抗生素和培养基的混合物。在全身麻醉下每日抽取胰液。在14天的观察中,除1例导管脱位外,无其他并发症发生。每天1毫升果汁的平均量已被证明足以用于各种酶的估计以及果汁细胞学。
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[A technique for pancreatic juice collection in the rat].

Monitoring of exocrine graft function and pancreatic juice cytology have proved to be a reliable method of detection of pancreas allograft rejection. In order to confirm these clinical findings and to define further parameters, experimental work is still needed. The rat was chosen for these experiments and a technique of pancreatic juice procurement developed. In five animals a fully implantable closed reservoir-catheter system was used. For the prevention of bacterial contamination it was necessary to install a mixture of antibiotics and culture medium into the reservoir. Pancreatic juice was procured daily under general anaesthesia. During the observation time of 14 days no further complications were encountered with the exception of one catheter dislocation. The mean amount of juice of 1 cc per day has proved to be sufficient for various enzyme estimations as well as for juice cytology.

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