用DPPH(1,1-二苯-2-苦味酰肼)法研究中华白皮乙醇提取物的抗氧化活性

Putri Rahmayanti, Ridwanto Ridwanto
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摘要

中国贝类植物(Leucaena leucocephala (Lam.) de Wit)已在各种传统医学系统中用于治疗人类的各种疾病。中国贝类植物(Leucaena leucocephala (Lam.) de Wit)含有抗氧化次生代谢物,可以通过与自由基和高活性分子结合来抑制氧化反应。本研究的目的是测定中华贝皮乙醇提取物(EEKPC)的化学成分及其抗氧化活性。本研究的阶段包括:单纯体的收集和处理;70%乙醇浸渍法制备提取物;茯苓粉的表征试验;牡丹乙醇提取物的植物化学筛选采用紫外-可见分光光度法测定EEKPC的抗氧化活性,波长516 nm。经植物化学筛选,黄芩醇提物中含有生物碱、黄酮类、皂苷、单宁和三萜。测定结果显示,EEKPC的抗氧化活性为“强”,IC50值为91.0189 μg/ml。
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Antioxidant Activity of Ethanol Extract of Chinese Petai Peel (Leucaena leucocephala (Lam.) de Wit) Using DPPH (1,1-diphenil-2-picrylhydrazyl) Method
The Chinese petai plant (Leucaena leucocephala (Lam.) de Wit) has been used in various systems of traditional medicine for the treatment of various diseases in humans. The Chinese petai plant (Leucaena leucocephala (Lam.) de Wit) contains antioxidant secondary metabolites that can inhibit oxidation reactions by binding to free radicals and highly reactive molecules. The purpose of this study was to determine the class of chemical compounds contained in the ethanol extract of Chinese petai peel and the antioxidant activity of the ethanol extract of Chinese petai peel (EEKPC). The stages of this research included: collection and processing of simplicia; preparation of the extract by maceration with 70% ethanol; characterization test of simplicia powder; phytochemical screening of ethanol extract of petai cina; antioxidant activity test of EEKPC using the DPPH (1,1-diphenyl-2-pikrilhidrazyl) free radical scavenging method using UV-Vis spectrophotometry at a wavelength of 516 nm. The results of the phytochemical screening examination of Chinese Petai Peel Ethanol Extract (EEKPC) contained alkaloids, flavonoids, saponins, tannins, and triterpenoids. The results of measuring the antioxidant activity of EEKPC showed strength in the "strong" category with an IC50 value of 91.0189 μg/ml.
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