Isolation, purification and identification of bacterial wilt pathogen from rhizosphere of Solanum melongena of Hadoti region

Solanum melongena L. (Brinjal), quite common and popular vegetable crop grown extensively during warm weather in India and is a major source of income for the small and marginal farmers as well. The major constraint in the production of brinjal is the bacterial wilt disease caused by Ralstonia solanacearum. Crop rotation with non-host plants has not been effective, since R. solanacearum has its disseminating and survival stages in the soil. The race and strain diversity of the pathogen has made breeding for resistant cultivars ineffective. That’s why study was done to isolate wilt causing bacteria Ralstonia solanacearum from the infected roots of Brinjal plant of Hadoti region and characterize the isolated bacteria through various methods. Initial cultures were grown by streaking on nutrient agar medium. After, bacterial wilt pathogen Ralstonia solanacearum was identified; it was confirmed by PCR (Polymerase chain reaction) amplification. Isolate of R. solanacearum were characterized by various DNA Markers.