哺乳动物水通道蛋白生物学研究方法

IF 2.5 Q3 BIOCHEMICAL RESEARCH METHODS Biology Methods and Protocols Pub Date : 2023-11-11 DOI:10.1093/biomethods/bpad031
Shohini Banerjee, Ian M Smith, Autumn C Hengen, Kimberly M Stroka
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引用次数: 0

摘要

摘要水通道蛋白(Aquaporins, AQPs)是一种跨膜的水传导通道,因其在健康和疾病细胞状态中的重要生理作用而受到广泛关注,特别是在生物医学领域。许多方法已经被用于阐明aqp介导的水转运和下游信号激活在引发整个细胞、组织和器官功能反应中的作用。为了调节这些反应,研究人员采用了其他方法来研究AQP的药物作用。本文综述了aqp在体外、体内和计算机上的标准研究方法,特别是在生物医学和哺乳动物细胞生物学方面的应用。我们还为未来AQP研究提出了一些新的技术和方法,以解决目前方法上的差距。
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Methods for Studying Mammalian Aquaporin Biology
Abstract Aquaporins (AQPs), transmembrane water-conducting channels, have earned a great deal of scrutiny for their critical physiological roles in healthy and disease cell states, especially in the biomedical field. Numerous methods have been implemented to elucidate the involvement of AQP-mediated water transport and downstream signaling activation in eliciting whole cell, tissue, and organ functional responses. To modulate these responses, other methods have been employed to investigate AQP druggability. This review discusses standard in vitro, in vivo, and in silico methods for studying AQPs, especially for biomedical and mammalian cell biology applications. We also propose some new techniques and approaches for future AQP research to address current gaps in methodology.
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来源期刊
Biology Methods and Protocols
Biology Methods and Protocols Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
3.80
自引率
2.80%
发文量
28
审稿时长
19 weeks
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