溴脱氧尿苷免疫组织化学用于气管伤口愈合的定位。

E M McDowell, X M Zhang, A M DeSanti
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引用次数: 15

摘要

再生细胞中胸腺嘧啶类似物溴脱氧尿嘧啶(BrdU)的免疫组织化学证明有助于确定仓鼠气管上皮再生过程中受伤区域(上皮和粘膜下)的大小和位置,有时在愈合过程后期(损伤后72-144小时),当常规染色石蜡切片无法确定伤口位置及其边界时。远离伤口部位的细胞仍未被标记。该方法的成功是由于在损伤后24小时皮下植入25mg片剂,在几个小时内释放BrdU,这是DNA合成和细胞增殖最大的时间。这项简单的技术有望用于确定伤口部位的大小和位置,并在修复和愈合的研究中广泛应用于各种器官和组织。
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Localization of healing tracheal wounds using bromodeoxyuridine immunohistochemistry.

Immunohistochemical demonstration of the thymidine analogue bromodeoxyuridine (BrdU) in regenerating cells was useful in determining the size and location of the wounded areas (epithelial and submucosal) during regeneration of the hamster tracheal epithelium, at times late in the healing process (72-144 hr postinjury) when the wound sites and their boundaries were not recognized with certainty in conventionally stained paraffin sections. Cells distant from the wound sites remained unlabelled. The success of this method resulted from prolonged exposure to BrdU released over several hours from a 25mg tablet implanted subcutaneously at 24 hr postwounding at the time when DNA synthesis and cell proliferation are maximal. This simple technique promises to be useful in determining the size and location of wound sites with application to a wide variety of organs and tissues in studies of repair and healing.

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