重度吸烟者血液淋巴细胞表面抗原和细胞毒性自然杀伤细胞活性的研究。

Archiv fur Geschwulstforschung Pub Date : 1990-01-01
E Jezewska, G Dworacki, A Skrzypczak, J Zeromski
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引用次数: 0

摘要

测定了重度吸烟者和非吸烟者对照组外周血白细胞淋巴细胞的表面表型和细胞毒性NK活性。通过间接免疫荧光法在细胞沉积物上检测单克隆抗体,评估细胞表型。以k562细胞为靶细胞,采用单细胞毒试验评价细胞毒活性。T细胞(cd3 +)和cd43 +(大唾液糖蛋白)的百分比值没有显著差异。然而,吸烟者中含有羊红细胞受体(cd2 +)的细胞比不吸烟者多。治疗组B淋巴细胞百分率明显低于对照组。在两个检查组中,激活细胞和未成熟细胞的百分比值没有差异。与吸烟者相比,不吸烟者NK细胞活性的百分比值更高。这反映在效应细胞的细胞毒性增加,而NK细胞的发生率在两组检测中是相似的。
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Surface antigens and cytotoxic natural killer cell (NK) activity of blood lymphocytes in heavy cigarette smokers.

Surface phenotypes of lymphocytes and the assessment of cytotoxic NK activity were determined in peripheral blood leukocytes in a group of heavy smokers and respective non-smoking people control group. Cell phenotypes were evaluated by a panel of monoclonal antibodies by indirect immunofluorescence on cell sediments. Cytotoxic activity was assessed by single cell cytotoxic assay on target K 562 cells. There were no significant differences in T cell (CD 3+) as well as in CD 43+ ones (large sialoglycoprotein) per cent values. The cells possessing receptor for sheep red blood cell (CD 2+) were however more numerous in smokers as compared to non-smokers. Per cent value of B lymphocytes in the former group was significantly decreased vs control one. There was no difference in per cent values of activated and immature cells in both examined groups. Per cent values of NK cell activity were higher in non-smokers in relation to smokers. It was reflected by an increase of cytotoxicity of effector cells, while frequency of incidence of NK cells was comparable in both groups examined.

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