真菌介导的聚合物在环境系统(垃圾场)中的生物降解途径探索

None Olaide Z., None Patricia O. N., None Esther C. A., None Obiefoka S. O., None Temitope S., None Mustapha S., None Adeyemi A. K., None Yahaya R. A.
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引用次数: 0

摘要

近几十年来,越来越多的环境塑料引起了越来越多的关注和担忧。虽然易于生产,耐用性,成本效益和耐腐蚀性是消费品的重要属性,但它们有助于持久的环境存在,导致塑料污染的广泛激增。目的:探索各种微生物介导的聚合物(黑尼龙和白尼龙)的生物降解途径。设置与设计:选取Oyo州Ibadan的一个机械垃圾场和一个医院垃圾场的排土场进行研究。 材料与方法:取排土场土,进行稀释(连续)和培养,并进行形态学和显微镜观察,同时在第5、10、15和20天降解黑色尼龙和白色尼龙塑料袋。 结果:聚合物的生物降解受到聚合物特性和环境中特定微生物存在的双重影响。在垃圾场中发现了黑曲霉、黄曲霉和真菌等菌种。几天来对聚乙烯袋(黑色尼龙和白色尼龙)的重量分析显示出轻微的变化,表明有一些降解。PCR分析证实了真菌分离物的遗传物质,突出了所选引物的特异性和敏感性。 结论:曲霉在垃圾场的存在表明它们在具有挑战性的环境中具有生物修复的潜力
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Exploring Fungi-mediated Biodegradation Pathway of Polymers in Environmental Systems(Dump Sites)
Context: The growing presence of environmental plastic has garnered escalating attention and worry in recent decades. While ease of production, durability, cost-effectiveness, and resistance to corrosion are valued attributes for consumer goods, they contribute to the persistent environmental presence, leading to a widespread surge in plastic pollution. Aim: To find out various microbial- mediated biodegradation pathway of polymers (black nylon and white nylon). Settings and Design: The research was carried out by obtaining dump soil from a mechanic dump site and a hospital dump site in Ibadan, Oyo state. Materials and Methodology: Dump soil were obtained, diluted(serial) and Cultered and morphological and microscopic observation were carried out while polythene bags which are black nylon and white nylon were also degraded at Day 5, 10 ,15 and 20. Results: Biodegradation of polymers is influenced by both polymer characteristics and the presence of specific microorganisms in the environment. Fungi, with species like A. niger, A. flavus, and C. fungus were identified in a dump site. Weight analysis of polythene bags (black nylon and white nylon) over days revealed slight changes, indicating some degradation. PCR analysis confirmed genetic material from fungal isolates, highlighting the specificity and sensitivity of the chosen primers. Conclusion: The presence of Aspergillus species in the dump site suggests their potential for bioremediation efforts in challenging environments
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