AeiA是米曲霉中一种与atg8相互作用的蛋白,通过噬酶促进过氧化物酶体降解

Takashi Kikuma, Joichiro Nishio
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引用次数: 0

摘要

自噬有两种类型:非选择性(散装)自噬,其中底物被随机纳入自噬体;选择性自噬,其中底物被特异性靶向。在丝状真菌中,选择性自噬的分子机制在很大程度上仍然未知。最近,我们在丝状真菌米曲霉(Aspergillus oryzae)中发现了一种新的蛋白aoatg8相互作用蛋白a (AeiA)。AeiA定位于过氧化物酶体和自噬体中间体,如吞噬体组装位点(PAS)和吞噬体。此外,AeiA颗粒的自噬通量降低。综上所述,AeiA是一种新的选择性自噬相关蛋白,参与了m.o ryzae的自噬。我们的研究结果为丝状真菌选择性自噬的分子机制提供了新的见解。缩写:AIM, atg8 -家族相互作用基序;at8,自噬相关的8;增强型绿色荧光蛋白;GABARAP, γ氨基丁酸A受体相关蛋白;LC3,微管相关蛋白轻链3;微体靶向信号;PD,马铃薯葡萄糖。
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AeiA, an Atg8-interacting protein in Aspergillus oryzae , promotes peroxisome degradation by pexophagy
There are two types of autophagy, non-selective (bulk) autophagy, in which substrates are randomly incorporated into autophagosomes, and selective autophagy, in which substrates are specifically targeted. In filamentous fungi, the molecular mechanism underlying selective autophagy remains largely unknown. Recently we identified a novel protein, AoAtg8-interacting protein A (AeiA), in the filamentous fungus Aspergillus oryzae. AeiA was localized to peroxisomes and autophagosomal intermediates, such as phagophore assembly site (PAS) and the phagophore. Moreover, pexophagy flux was reduced in AeiA deletants. Taken together, AeiA is a novel selective autophagy-related protein that contributes to pexophagy in A. oryzae. Our findings provide insight into the molecular mechanisms of selective autophagy including pexophagy in filamentous fungi. Abbreviations: AIM, Atg8-family interacting motifs; Atg8, autophagy-related 8; EGFP, enhanced green fluorescent protein; GABARAP, Gamma aminobutyric acid A receptor associated protein; LC3, Microtubule-associated protein light chain 3; MTS, microbody targeting signal; PD, potato dextrose.
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