{"title":"山楂叶提取物对鱼类致病菌的抑制作用","authors":"Jarod Setiaji, Hisra Melati, Muchtar Achmad, None Heriyanto, Valentio Febian Prokoso, Tengku Said Raza’i, Rahmat Huluan, Reyza Pramadani","doi":"10.1051/bioconf/20237001005","DOIUrl":null,"url":null,"abstract":"Bacterial diseases have emerged as a significant issue within the fish ecosystem, and the use of antibiotics to treat these diseases has detrimental effects on both fish and the environment. Therefore, it is imperative to seek out natural products that are safe for combating fish diseases. This research aims to assess the antibacterial properties of an extract derived from Muntingia calabura against various bacteria, including A. hydrophila, A. salmonicida, E. ictaluri, P. aeruginosa, E. tarda, and V. alginolyticus. M. calabura extract was obtained by soaking with ethanol solvent. This extract has been tested for phytochemical content and characterized by FT-IR. The antibacterial activity of the M. calabura extract was evaluated using the agar diffusion method, employing concentrations of 100, 200, 300, 400, and 500 mg mL-1. The phytochemical analysis of the M. calabura extract revealed the presence of flavonoids, phenolic compounds, saponins, and terpenoids. The results of the FT-IR characterization of M. calabura extract contained O-H, C=O, C=C aliphatic, C=C aromatic and C-O groups. The inhibition tests demonstrated that the M. calabura extract effectively hindered the growth of A. hydrophila bacteria by a range of 8.1 mm to 14.2 mm, A. salmonicida by 9.2 mm to 10.9 mm, E. ictaluri by 8.8 mm to 11.5 mm, P. aeruginosa by 8.1 mm to 12.1 mm, E. tarda by 9.6 mm to 11.4 mm, and V. alginolyticus by 8.2 mm to 12.2 mm. In summary, the extract from M. calabura has the potential to effectively on paper plates and incubated at 30°C for 24 hours.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The activity of Muntingia calabura leaf extract against pathogenic bacteria in fish\",\"authors\":\"Jarod Setiaji, Hisra Melati, Muchtar Achmad, None Heriyanto, Valentio Febian Prokoso, Tengku Said Raza’i, Rahmat Huluan, Reyza Pramadani\",\"doi\":\"10.1051/bioconf/20237001005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Bacterial diseases have emerged as a significant issue within the fish ecosystem, and the use of antibiotics to treat these diseases has detrimental effects on both fish and the environment. Therefore, it is imperative to seek out natural products that are safe for combating fish diseases. This research aims to assess the antibacterial properties of an extract derived from Muntingia calabura against various bacteria, including A. hydrophila, A. salmonicida, E. ictaluri, P. aeruginosa, E. tarda, and V. alginolyticus. M. calabura extract was obtained by soaking with ethanol solvent. This extract has been tested for phytochemical content and characterized by FT-IR. The antibacterial activity of the M. calabura extract was evaluated using the agar diffusion method, employing concentrations of 100, 200, 300, 400, and 500 mg mL-1. The phytochemical analysis of the M. calabura extract revealed the presence of flavonoids, phenolic compounds, saponins, and terpenoids. The results of the FT-IR characterization of M. calabura extract contained O-H, C=O, C=C aliphatic, C=C aromatic and C-O groups. The inhibition tests demonstrated that the M. calabura extract effectively hindered the growth of A. hydrophila bacteria by a range of 8.1 mm to 14.2 mm, A. salmonicida by 9.2 mm to 10.9 mm, E. ictaluri by 8.8 mm to 11.5 mm, P. aeruginosa by 8.1 mm to 12.1 mm, E. tarda by 9.6 mm to 11.4 mm, and V. alginolyticus by 8.2 mm to 12.2 mm. In summary, the extract from M. calabura has the potential to effectively on paper plates and incubated at 30°C for 24 hours.\",\"PeriodicalId\":8805,\"journal\":{\"name\":\"BIO Web of Conferences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BIO Web of Conferences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1051/bioconf/20237001005\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BIO Web of Conferences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1051/bioconf/20237001005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The activity of Muntingia calabura leaf extract against pathogenic bacteria in fish
Bacterial diseases have emerged as a significant issue within the fish ecosystem, and the use of antibiotics to treat these diseases has detrimental effects on both fish and the environment. Therefore, it is imperative to seek out natural products that are safe for combating fish diseases. This research aims to assess the antibacterial properties of an extract derived from Muntingia calabura against various bacteria, including A. hydrophila, A. salmonicida, E. ictaluri, P. aeruginosa, E. tarda, and V. alginolyticus. M. calabura extract was obtained by soaking with ethanol solvent. This extract has been tested for phytochemical content and characterized by FT-IR. The antibacterial activity of the M. calabura extract was evaluated using the agar diffusion method, employing concentrations of 100, 200, 300, 400, and 500 mg mL-1. The phytochemical analysis of the M. calabura extract revealed the presence of flavonoids, phenolic compounds, saponins, and terpenoids. The results of the FT-IR characterization of M. calabura extract contained O-H, C=O, C=C aliphatic, C=C aromatic and C-O groups. The inhibition tests demonstrated that the M. calabura extract effectively hindered the growth of A. hydrophila bacteria by a range of 8.1 mm to 14.2 mm, A. salmonicida by 9.2 mm to 10.9 mm, E. ictaluri by 8.8 mm to 11.5 mm, P. aeruginosa by 8.1 mm to 12.1 mm, E. tarda by 9.6 mm to 11.4 mm, and V. alginolyticus by 8.2 mm to 12.2 mm. In summary, the extract from M. calabura has the potential to effectively on paper plates and incubated at 30°C for 24 hours.